Scopoletin 8-hydroxylase: a novel enzyme involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis

Joanna Siwińska , Kinga Siatkowska , Alexandre Olry , Jeremy Grosjean , Alain Hehn , Frederic Bourgaud , Andrew A. Meharg , Manus Carey , Ewa Łojkowska , Anna Ihnatowicz

Abstract

Iron deficiency is a serious agricultural problem, particularly in alkaline soils. Secretion of coumarins by Arabidopsis thaliana roots is induced under iron deficiency. An essential enzyme for the biosynthesis of the major Arabidopsis coumarins, scopoletin and its derivatives, is Feruloyl-CoA 6′-Hydroxylase1 (F6′H1), which belongs to a large enzyme family of the 2-oxoglutarate and Fe2+-dependent dioxygenases. We have functionally characterized another enzyme of this family, which is a close homologue of F6′H1 and is encoded by a strongly iron-responsive gene, At3g12900. We purified At3g12900 protein heterologously expressed in Escherichia coli and demonstrated that it is involved in the conversion of scopoletin into fraxetin, via hydroxylation at the C8 position, and that it thus functions as a scopoletin 8-hydroxylase (S8H). Its function in plant cells was confirmed by the transient expression of S8H protein in Nicotiana benthamiana leaves, followed by metabolite profiling and biochemical and ionomic characterization of Arabidopsis s8h knockout lines grown under various iron regimes. Our results indicate that S8H is involved in coumarin biosynthesis, as part of mechanisms used by plants to assimilate iron.
Author Joanna Siwińska KB
Joanna Siwińska,,
- Department of Biotechnology
, Kinga Siatkowska MWB UG i GUMed
Kinga Siatkowska,,
- Intercollegiate Faculty of Biotechnology UG
, Alexandre Olry
Alexandre Olry,,
-
, Jeremy Grosjean
Jeremy Grosjean,,
-
, Alain Hehn
Alain Hehn,,
-
, Frederic Bourgaud
Frederic Bourgaud,,
-
, Andrew A. Meharg
Andrew A. Meharg,,
-
, Manus Carey
Manus Carey,,
-
, Ewa Łojkowska KB
Ewa Łojkowska,,
- Department of Biotechnology
, Anna Ihnatowicz KB
Anna Ihnatowicz,,
- Department of Biotechnology
Journal seriesJournal of Experimental Botany, ISSN 0022-0957
Issue year2018
Vol69
No7
Pages1735-1748
Publication size in sheets0.65
Keywords in Englishabiotic stress, Arabidopsis, enzyme activity, fraxetin, Fe- and 2OG-dependent dioxygenase, mineral nutrition, plant-environment interactions
DOIDOI:10.1093/jxb/ery005
URL https://doi.org/10.1093/jxb/ery005
Languageen angielski
Score (nominal)45
ScoreMinisterial score = 45.0, 30-05-2018, ArticleFromJournal
Ministerial score (2013-2016) = 45.0, 30-05-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 5.83 (2) - 2016=6.538 (5)
Citation count*0
Cite
Share Share

Get link to the record
msginfo.png


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back