Changes in nitric oxide/hydrogen peroxide content and cell cycle progression: study with synchronized cultures of green alga Chlamydomonas reinhardtii

Wojciech Pokora , Anna Aksmann , Agnieszka Baścik-Remisiewicz , Agnieszka Dettlaff-Pokora , Max Rykaczewski , Magdalena Gappa , Zbigniew Tukaj

Abstract

The present study aimed to evaluate the possible relationship between the changes in hydrogen peroxide (H2O2) and nitric oxide (NO) content and the course of growth and reproductive processes of the cell cycle of Chlamydomonas reinhardtii. The peak of H2O2 observed at the beginning of the cell cycle was found to originate from Fe-SOD and Mn-SODchl. activity and result from the alternation in the photosynthetic processes caused by the dark-to-light transition of daughter cells. A rapid increase in NO concentration, observed before the light-to-dark cell transition, originated from NR and NIR activity and was followed by a photosynthesis-independent, Mn-SODchl.-mediated increases in H2O2 production. This H2O2 peak overlapped the beginning of Chlamydomonas cell division, which was indicated by a profile of CYCs and CDKs characteristic of cells’ passage through the G1/S and S/M checkpoints. Taken together, our results show that there is a clear relationship between the course of the Chlamydomonas cell cycle and typical changes in the H2O2/NO ratio, as well as changes in expression and activity of enzymes involved in generation and scavenging of these signaling molecules.
Author Wojciech Pokora (FB / DPPB)
Wojciech Pokora,,
- Department of Plant Physiology and Biotechnology
, Anna Aksmann (FB / DPPB)
Anna Aksmann,,
- Department of Plant Physiology and Biotechnology
, Agnieszka Baścik-Remisiewicz (FB / DPPB)
Agnieszka Baścik-Remisiewicz,,
- Department of Plant Physiology and Biotechnology
, Agnieszka Dettlaff-Pokora
Agnieszka Dettlaff-Pokora,,
-
, Max Rykaczewski (FB / DPTNC)
Max Rykaczewski,,
- Department of Plant Taxonomy and Nature Conservation
, Magdalena Gappa (FB)
Magdalena Gappa,,
- Faculty of Biology
, Zbigniew Tukaj (FB / DPPB)
Zbigniew Tukaj,,
- Department of Plant Physiology and Biotechnology
Journal seriesJournal of Plant Physiology, ISSN 0176-1617, (A 35 pkt)
Issue year2017
Vol208
Pages84-93
Publication size in sheets0.5
Keywords in EnglishChlamydomonas, nitric oxide, hydrogen peroxide, cell cycle
DOIDOI:10.1016/j.jplph.2016.10.008
URL http://dx.doi.org/10.1016/j.jplph.2016.10.008
Languageen angielski
Score (nominal)35
ScoreMinisterial score = 35.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 3.121 (2) - 2016=3.296 (5)
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