Metody chemicznej ligacji w syntezie peptydów i białek - część I

Magdalena Kropidłowska , Katarzyna Jędrzejewska , Ewa Wieczerzak

Abstract

Proteins are biological macromolecules affecting very important functions in the body. They are involved in many biochemical processes. They can perform catalytic functions acting as enzymes. They also participate in the transport of many small molecules and ions – for example one molecule of hemoglobin carries four molecules of oxygen. In addition, proteins serve as antibodies and are involved in transmission of nerve impulses as receptor proteins. Because peptides and proteins perform so important functions, to study them it is essential to obtain these compounds in the greatest possible amounts. The compounds can be obtained generally by three main methods: • by isolation of the native peptides and proteins • by expression in microorganisms • by chemical synthesis. Each of the above methods has its advantages and disadvantages, but only the chemical synthesis gives the possibility to introduce modifications to the structure of the resulting protein, such as the insertion of new functional groups, to give the product in the final form and with satisfactory yield. In this review we present the application of chemical ligation methods in the synthesis of peptides and proteins. We describe in details mechanism of native chemical ligation method and the conditions necessary to carry the reaction [1]. The synthesis of long polypeptide chains by kinetically controlled ligation (KCL) is also depicted [2]. This part of the paper also details a number of approaches to noncysteine containing peptides by chemical ligation methods.
Author Magdalena Kropidłowska PChM
Magdalena Kropidłowska ,,
- Laboratory of Medical Chemistry
, Katarzyna Jędrzejewska PChM
Katarzyna Jędrzejewska,,
- Laboratory of Medical Chemistry
, Ewa Wieczerzak PChM
Ewa Wieczerzak,,
- Laboratory of Medical Chemistry
Other language title versionsChemical ligation methods in the synthesis of peptides and proteins - part I
Journal seriesWiadomości Chemiczne, ISSN 0043-5104, e-ISSN 2300-0295
Issue year2017
Vol71
No9-10
Pages727-746
Publication size in sheets0.95
Keywords in Polishnatywna chemiczna ligacja, NCL, kinetycznie kontrolowana ligacja, KCL, synteza peptydów
Keywords in Englishnative chemical ligation, NCL, kinetically controlled ligation, KCL, peptide synthesis
URL http://wiad.chem.uni.wroc.pl/files/WCh_9-10-2017_DBC3.pdf
Languagepl polski
LicenseOther; published final; Other open licence; with publication
Score (nominal)7
ScoreMinisterial score = 7.0, 26-02-2018, ArticleFromJournal
Ministerial score (2013-2016) = 7.0, 26-02-2018, ArticleFromJournal
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