Exogenously applied hydrogen peroxide modifies the course of the Chlamydomonas reinhardtii cell cycle

Wojciech Pokora , Anna Aksmann , Agnieszka Baścik-Remisiewicz , Agnieszka Dettlaff-Pokora , Zbigniew Tukaj

Abstract

The interaction of NO and H2O2 in the regulation of plant development is well documented. We have recently shown that the content of NO and H2O2 changes in a characteristic way during the cell cycle of Chlamydomonas reinhardtii (Pokora et al., 2017), which implies participation of these molecules in the regulation of Chlamydomonas development. To verify this assumption, H2O2 was supplied at a concentration about 1.5 times higher than that determined in the control cells. Cells were synchronized by alternating the light/dark (10/14 h) regimen. H2O2 was added to zoospore suspensions, previously held in the dark, and cells growing for 3, 6, and 9 h in the light. The data indicate that, depending on the phase of the Chlamydomonas cell cycle, H2O2, via mild modification of redox homeostasis, may: a) accelerate or delay the duration of the cell cycle; b) increase the number of replication rounds occurring in one cell cycle; c) modify the biomass and cell volume of progeny cells and d) accelerate the liberation of daughter cells. This provides a tool to control the development of Chlamydomonas cell and thus offers the opportunity to obtain a population of cells with characteristics desired in biotechnology.
Author Wojciech Pokora (FB / DPPB)
Wojciech Pokora,,
- Department of Plant Physiology and Biotechnology
, Anna Aksmann (FB / DPPB)
Anna Aksmann,,
- Department of Plant Physiology and Biotechnology
, Agnieszka Baścik-Remisiewicz (FB / DPPB)
Agnieszka Baścik-Remisiewicz,,
- Department of Plant Physiology and Biotechnology
, Agnieszka Dettlaff-Pokora
Agnieszka Dettlaff-Pokora,,
-
, Zbigniew Tukaj (FB / DPPB)
Zbigniew Tukaj,,
- Department of Plant Physiology and Biotechnology
Journal seriesJournal of Plant Physiology, ISSN 0176-1617, (A 35 pkt)
Issue year2018
Vol230
Pages61-72
Publication size in sheets0.55
Keywords in EnglishChlamydomonas, cell cycle, hydrogen peroxide, nitric oxide, redox homeostasis
DOIDOI:10.1016/j.jplph.2018.07.015
URL https://doi.org/10.1016/j.jplph.2018.07.015
Languageen angielski
Score (nominal)35
ScoreMinisterial score = 35.0, 11-09-2018, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 11-09-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 3.121 (2) - 2016=3.296 (5)
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