Multiple links connect central carbon metabolism to DNA replication initiation and elongation in Bacillus subtilis

Hamid Nouri , Anne-Françoise Monnier , Solveig Fossum-Raunehaug , Monika Maciąg-Dorszyńska , Armelle Cabin-Flaman , François Képès , Grzegorz Węgrzyn , Agnieszka Szalewska-Pałasz , Vic Norris , Kirsten Skarstad , Laurent Janniere

Abstract

DNA replication is coupled to growth by an unknown mechanism. Here, we investigated this coupling by analyzing growth and replication in 15 mutants of central carbon metabolism (CCM) cultivated in three rich media. In about one-fourth of the condition tested, defects in replication resulting from changes in initiation or elongation were detected. This uncovered 11 CCM genes important for replication and showed that some of these genes have an effect in one, two or three media. Additional results presented here and elsewhere (Jannière, L., Canceill, D., Suski, C., et al. (2007), PLoS One, 2, e447.) showed that, in the LB medium, the CCM genes important for DNA elongation (gapA and ackA) are genetically linked to the lagging strand polymerase DnaE while those important for initiation (pgk and pykA) are genetically linked to the replication enzymes DnaC (helicase), DnaG (primase) and DnaE. Our work thus shows that the coupling between growth and replication involves multiple, medium-dependent links between CCM and replication. They also suggest that changes in CCM may affect initiation by altering the functional recruitment of DnaC, DnaG and DnaE at the chromosomal origin, and may affect elongation by altering the activity of DnaE at the replication fork. The underlying mechanism is discussed.
Publication typeIn press (online first, early view)
Author Hamid Nouri
Hamid Nouri,,
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, Anne-Françoise Monnier
Anne-Françoise Monnier,,
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, Solveig Fossum-Raunehaug
Solveig Fossum-Raunehaug,,
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, Monika Maciąg-Dorszyńska (FB / DMGB)
Monika Maciąg-Dorszyńska,,
- Department of Molecular Genetics of Bacteria
, Armelle Cabin-Flaman
Armelle Cabin-Flaman,,
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, François Képès
François Képès,,
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, Grzegorz Węgrzyn (FB / DMoB)
Grzegorz Węgrzyn,,
- Department of Molecular Biology
, Agnieszka Szalewska-Pałasz (FB / DMGB)
Agnieszka Szalewska-Pałasz,,
- Department of Molecular Genetics of Bacteria
, Vic Norris
Vic Norris,,
-
, Kirsten Skarstad
Kirsten Skarstad,,
-
et al.`
Journal seriesDNA Research, ISSN 1340-2838, (A 40 pkt)
Issue year2018
Noonline first
Pages1-13
Publication size in sheets0.6
Keywords in Englishcell cycle, DNA replication, metabolism, signalling, integrative biology
DOIDOI:10.1093/dnares/dsy031
URL https://doi.org/10.1093/dnares/dsy031
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa - Użycie Niekomercyjne (CC-BY-NC); with publication
Score (nominal)40
ScoreMinisterial score = 40.0, 29-10-2018, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 29-10-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 5.404 (2) - 2016=5.524 (5)
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