Multiple links connect central carbon metabolism to DNA replication initiation and elongation in Bacillus subtilis

Hamid Nouri , Anne-Françoise Monnier , Solveig Fossum-Raunehaug , Monika Maciąg-Dorszyńska , Armelle Cabin-Flaman , François Képès , Grzegorz Węgrzyn , Agnieszka Szalewska-Pałasz , Vic Norris , Kirsten Skarstad , Laurent Janniere


DNA replication is coupled to growth by an unknown mechanism. Here, we investigated this coupling by analyzing growth and replication in 15 mutants of central carbon metabolism (CCM) cultivated in three rich media. In about one-fourth of the condition tested, defects in replication resulting from changes in initiation or elongation were detected. This uncovered 11 CCM genes important for replication and showed that some of these genes have an effect in one, two or three media. Additional results presented here and elsewhere (Jannière, L., Canceill, D., Suski, C., et al. (2007), PLoS One, 2, e447.) showed that, in the LB medium, the CCM genes important for DNA elongation (gapA and ackA) are genetically linked to the lagging strand polymerase DnaE while those important for initiation (pgk and pykA) are genetically linked to the replication enzymes DnaC (helicase), DnaG (primase) and DnaE. Our work thus shows that the coupling between growth and replication involves multiple, medium-dependent links between CCM and replication. They also suggest that changes in CCM may affect initiation by altering the functional recruitment of DnaC, DnaG and DnaE at the chromosomal origin, and may affect elongation by altering the activity of DnaE at the replication fork. The underlying mechanism is discussed.
Author Hamid Nouri - [Institut de Biologie Systémique et Synthétique]
Hamid Nouri,,
, Anne-Françoise Monnier - [Microbiologie de l'Alimentation au Service de la Santé Humaine]
Anne-Françoise Monnier,,
, Solveig Fossum-Raunehaug - [Oslo University Hospital]
Solveig Fossum-Raunehaug,,
, Monika Maciąg-Dorszyńska (FB/DMGB)
Monika Maciąg-Dorszyńska,,
- Department of Molecular Genetics of Bacteria
, Armelle Cabin-Flaman - [AMMIS]
Armelle Cabin-Flaman,,
, François Képès
François Képès,,
, Grzegorz Węgrzyn (FB/DMoB)
Grzegorz Węgrzyn,,
- Department of Molecular Biology
, Agnieszka Szalewska-Pałasz (FB/DMGB)
Agnieszka Szalewska-Pałasz,,
- Department of Molecular Genetics of Bacteria
, Vic Norris - [Uniwersytet Gdanski]
Vic Norris,,
- Uniwersytet Gdanski
, Kirsten Skarstad - [AMMIS]
Kirsten Skarstad,,
et al.`
Journal seriesDNA Research, ISSN 1340-2838, (A 40 pkt)
Issue year2018
Publication size in sheets0.60
Keywords in Englishcell cycle, DNA replication, metabolism, signalling, integrative biology
ASJC Classification2700 General Medicine; 1311 Genetics; 1312 Molecular Biology
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa - Użycie Niekomercyjne (CC-BY-NC); with publication
Score (nominal)40
Score sourcejournalList
ScoreMinisterial score = 40.0, 10-07-2020, ArticleFromJournal
Publication indicators WoS Citations = 2.000; Scopus Citations = 3.000; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.460; WoS Impact Factor: 2018 = 4.000 (2) - 2018=5.016 (5)
Citation count*5 (2020-06-29)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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