Effects of stressful physico-chemical factors on the fitness of the plant pathogenic bacterium Dickeya solani

Tomasz Przepióra , Donata Figaj , Marta Radzińska , Małgorzata Apanowicz , Małgorzata Sieradzka , Patrycja Ambroziak , Nicole Hugouvieux-Cotte-Pattat , Ewa Łojkowska , Joanna Skórko-Glonek

Abstract

Dickeya solani is a pectinolytic bacterium that causes significant losses of potato crops. Interaction between bacteria and plant during infection and transmission within the host tissue are associated with a variety of challenges, including exposure to physico-chemical factors that induce osmotic, acidic, oxidative or thermal stresses. In this work, we tested the effects of various potentially adverse conditions on growth and fitness of the D. solani IPO2222 type strain. We found the bacteria were able to withstand a great variety of stressful conditions. Treatment with high concentrations of osmolytes (up to 1.6 Osm), low pH (5.0), or elevated temperature (up to 40 °C) was still tolerated. Short term exposure to these conditions did not impair the ability of bacteria to macerate potato tuber tissue. However, D. solani was sensitive to a relatively low content (above 0.5 mM) of the oxidant H2O2 and treatment with this oxidant caused a loss of culturability. All used stressful conditions are known to affect the stability, and structure of proteins and induction of the folding stress-related genes, dnaK, dnaJ and groEL, was observed in most cases. The only exception was oxidative stress for which the chaperone genes were slightly down-regulated. This observation is in line with a low tolerance of D. solani to H2O2.
Author Tomasz Przepióra (FB/DB)
Tomasz Przepióra,,
- Department of Biochemistry
, Donata Figaj (FB/DB)
Donata Figaj,,
- Department of Biochemistry
, Marta Radzińska (FB)
Marta Radzińska,,
- Faculty of Biology
, Małgorzata Apanowicz (FB/DB)
Małgorzata Apanowicz,,
- Department of Biochemistry
, Małgorzata Sieradzka (FB/DB)
Małgorzata Sieradzka,,
- Department of Biochemistry
, Patrycja Ambroziak (FB)
Patrycja Ambroziak,,
- Faculty of Biology
, Nicole Hugouvieux-Cotte-Pattat
Nicole Hugouvieux-Cotte-Pattat,,
-
, Ewa Łojkowska (IFB/IB/DBiotech)
Ewa Łojkowska,,
- Department of Biotechnology
, Joanna Skórko-Glonek (FB/DB)
Joanna Skórko-Glonek,,
- Department of Biochemistry
Journal seriesEuropean Journal of Plant Pathology, ISSN 0929-1873, e-ISSN 1573-8469, (N/A 100 pkt)
Issue year2020
Vol156
No2
Pages519-535
Publication size in sheets0.80
Keywords in EnglishDickeya solani, potato pathogen, stress response, chaperones, infectivity
ASJC Classification1102 Agronomy and Crop Science; 1108 Horticulture; 1110 Plant Science
DOIDOI:10.1007/s10658-019-01902-z
URL https://doi.org/10.1007/s10658-019-01902-z
Languageen angielski
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 28-01-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.910; WoS Impact Factor: 2018 = 1.744 (2) - 2018=1.754 (5)
Citation count*1 (2020-06-26)
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