Compatible mixture of bacterial antagonists developed to protect potato tubers from Soft Rot caused by Pectobacterium spp. and Dickeya spp.

Dorota Krzyżanowska , Tomasz Maciąg , Joanna Siwińska , Marta Krychowiak , Sylwia Jafra , Robert Czajkowski

Abstract

Possibilities to protect potato tubers from rotting caused by Soft Rot Pectobacteriaceae (SRP) under disease favoring conditions were investigated using compatible mixtures of bacterial antagonists and tested with a newly developed stepwise efficacy-based screening protocol. Twenty-two bacterial antagonists were evaluated against a combination of five Pectobacterium and Dickeya strains representing species and subspecies most often associated with potato soft rot in Europe. To enable potential synergistic activity, the antagonists were initially tested against the combination of pathogens in 15 random mixtures containing up to 5 antagonists each. Three mixtures (M2, M4, and M14) out of 15 tested reduced tuber tissue maceration due to soft rot. The individual antagonists derived from M2, M4, and M14 mixtures were tested on potato slices and whole tuber injection assays. These five strains (S. plymuthica strain A294, E. amnigenus strain A167, R. aquatilis strain H145, S. rubidaea strain H440, and S. rubidaea strain H469) were combined to develop a tailored biological control mixture against potato soft rot. The new mixture, designated the Great Five (GF), was tested on seed potato tubers vacuum infiltrated with antagonists and subsequently with the combination of five SRP pathogens. In these experiments, the GF mixture provided stable protection of inoculated potato tubers, reducing soft rot by 46% (P = 0.0016) under high disease pressure conditions. The A294, A167, H145, H440, and H469 antagonists were characterized for features important for viable commercial applications including growth at different temperatures, resistance to antibiotics, and potential toxicity toward Caenorhabditis elegans. The implications for control of soft rot caused by SRP with the use of the GF mixture of antagonists are discussed.
Author Dorota Krzyżanowska (IFB / IB / DBiotech)
Dorota Krzyżanowska,,
- Department of Biotechnology
, Tomasz Maciąg (IFB)
Tomasz Maciąg,,
- Intercollegiate Faculty of Biotechnology UG
, Joanna Siwińska (IFB / IB / DBiotech)
Joanna Siwińska,,
- Department of Biotechnology
, Marta Krychowiak (IFB / IB / DBiotech)
Marta Krychowiak,,
- Department of Biotechnology
, Sylwia Jafra (IFB / IB / DBiotech)
Sylwia Jafra,,
- Department of Biotechnology
, Robert Czajkowski (IFB / IB / DBiotech)
Robert Czajkowski,,
- Department of Biotechnology
Journal seriesPlant Disease, ISSN 0191-2917, (A 35 pkt)
Issue year2019
Vol103
No6
Pages1374-1382
Publication size in sheets0.5
ASJC Classification1110 Plant Science; 1102 Agronomy and Crop Science
DOIDOI:10.1094/PDIS-10-18-1866-RE
URL https://doi.org/10.1094/PDIS-10-18-1866-RE
Languageen angielski
Score (nominal)35
ScoreMinisterial score = 35.0, 24-07-2019, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2017 = 0.984; WoS Impact Factor: 2017 = 2.941 (2) - 2017=3.469 (5)
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