AMCA to TAMRA long range resonance energy transfer on a flexible peptide

Anna Synak , Rafał Fudala , Ignacy Gryczyński , L. Kułak , S. Shah , Illia Serdiuk , Beata Grobelna , Piotr Arłukowicz , Aleksander Kubicki , Piotr Bojarski

Abstract

Förster resonance energy transfer between 7-amino-4-methyl-3-coumarinylacetic acid, (AMCA, donor) and 5- carboxytetramethylrhodamine, (TAMRA, acceptor) bound to Lys(AMCA)-Gly-Pro-Arg-Ser-Leu-Ser-Gly-Lys (TAMRA)-NH2 peptide is demonstrated by various spectroscopic techniques in glycerol at room temperature. In particular, nonexponential character of fluorescence intensity decay evidences the distance distribution between the donor and acceptor sites resulting from the flexibility of the peptide. Numerical analysis of the fluorescence decay yields the distance distribution parameters. The results obtained are consistent with those obtained from steady-state fluorescence indicating that energy transfer is more efficient than suggested by the relation between the critical distance and donor-acceptor distance for elongated peptide conformation.
Author Anna Synak IFD
Anna Synak,,
- Institute of Experimental Physics
, Rafał Fudala
Rafał Fudala,,
-
, Ignacy Gryczyński
Ignacy Gryczyński,,
-
, L. Kułak
L. Kułak,,
-
, S. Shah
S. Shah,,
-
, Illia Serdiuk IFD
Illia Serdiuk,,
- Institute of Experimental Physics
, Beata Grobelna PChiAK
Beata Grobelna,,
- Laboratory of chemistry and Analytics of Cosmetics
, Piotr Arłukowicz II
Piotr Arłukowicz,,
- Institute of Informatics
, Aleksander Kubicki IFD
Aleksander Kubicki,,
- Institute of Experimental Physics
, Piotr Bojarski IFD
Piotr Bojarski,,
- Institute of Experimental Physics
Journal seriesDyes and Pigments, ISSN 0143-7208
Issue year2018
Vol158
Pages60-64
Publication size in sheets0.5
DOIDOI:10.1016/j.dyepig.2018.05.019
URL https://doi.org/10.1016/j.dyepig.2018.05.019
Languageen angielski
Score (nominal)40
ScoreMinisterial score = 40.0, 14-06-2018, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 14-06-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 3.473 (2) - 2016=3.417 (5)
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