Dynamics of hepatitis B virus quasispecies heterogeneity in association with nucleos(t) ide analogue treatment determined by MALDI-TOF MS
Magda Rybicka , P. Stalke , Krzysztof Piotr Bielawski
AbstractMinor drug-resistant variants may preexist in every subject infected with hepatitis B virus (HBV). However, understanding the dynamic of genotypic evolution within the HBV population requires accurately following allele frequencies through time. We used MALDI-TOF MS (matrix-assisted laser desorption-ionization time-of- fl ight mass spectrometry) for localization and quantitative allele frequency detection to investigate preexisting HBV quasispecies and the genotypic evolution of drug-resistant variants during nucleos(t)ide analogue therapy. We found a signi fi cant difference between the genotypic evolution of drug- resistant variants depending on response to treatment.
|Journal series||Clinical Microbiology and Infection, ISSN 1198-743X, (A 40 pkt)|
|Publication size in sheets||0.3|
|Keywords in English||analogue, drug resistance, HBV, MALDI-TOF MS, quasispecies|
|License||Other; published final; ; after publication|
|Score|| = 45.0, 20-12-2017, ArticleFromJournal|
= 45.0, 20-12-2017, ArticleFromJournal
|Publication indicators||: 2015 = 4.575 (2) - 2015=4.782 (5)|
|Citation count*||4 (2018-12-18)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.