Dynamics of hepatitis B virus quasispecies heterogeneity in association with nucleos(t) ide analogue treatment determined by MALDI-TOF MS

Magda Rybicka , P. Stalke , Krzysztof Piotr Bielawski

Abstract

Minor drug-resistant variants may preexist in every subject infected with hepatitis B virus (HBV). However, understanding the dynamic of genotypic evolution within the HBV population requires accurately following allele frequencies through time. We used MALDI-TOF MS (matrix-assisted laser desorption-ionization time-of- fl ight mass spectrometry) for localization and quantitative allele frequency detection to investigate preexisting HBV quasispecies and the genotypic evolution of drug-resistant variants during nucleos(t)ide analogue therapy. We found a signi fi cant difference between the genotypic evolution of drug- resistant variants depending on response to treatment.
Author Magda Rybicka (IFB / IB / DBiotech)
Magda Rybicka,,
- Department of Biotechnology
, P. Stalke
P. Stalke,,
-
, Krzysztof Piotr Bielawski (IFB / IB / DBiotech)
Krzysztof Piotr Bielawski,,
- Department of Biotechnology
Journal seriesClinical Microbiology and Infection, ISSN 1198-743X, (A 40 pkt)
Issue year2015
Vol21
No3
Pages288.e1-288.e4
Publication size in sheets0.3
Keywords in Englishanalogue, drug resistance, HBV, MALDI-TOF MS, quasispecies
DOIDOI:10.1016/j.cmi.2014.10.004
URL http://dx.doi.org/10.1016/j.cmi.2014.10.004
Languageen angielski
LicenseOther; published final; Other open licence; after publication
Score (nominal)45
ScoreMinisterial score = 45.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 45.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2015 = 4.575 (2) - 2015=4.782 (5)
Citation count*4 (2018-12-18)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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