Phytotoxic activity of diclofenac: evaluation using a model green alga Chlamydomonas reinhardtii with atrazine as a reference substance

Monika Majewska , Darya Harshkova , Monika Guściora , Anna Aksmann

Abstract

Human activities have caused increasing inputs of pharmaceuticals to the environment and diclofenac (DF) is one of the most commonly detected in freshwater systems. The aim of this study was to determine the impact of DF on a freshwater green alga as a non-target organism. For DF toxicity evaluation, its effects on a model organism Chlamydomonas reinhardtii were compared with effects caused by the herbicide atrazine (AT). EC50 values were about 135 mg/L for DF and 78 mg/L for AT, respectively. Both toxicants enhanced H2O2 production by the cells (144% and 178% of control for AT and DF, respectively) and stimulated catalase activity (≈200% of control). Activity of ascorbate peroxidase was elevated in AT-cells but not in DF-treated cells. DF did not influence dark respiration of the cells, whereas AT inhibited this process by about 50% compared to the control. Both toxicants caused photosynthesis inhibition. Analysis of parameters of chlorophyll a fluorescence in vivo showed diminishment of a performance index (PI) in both DF- and AT-treated cells (≈50% of control), but the reasons for the changes detected were different. AT diminished the efficiency of electron transport between PS II and PS I without significant inhibition of PS II or PS I reaction centers (RCs). In contrast to AT, DF seemed to influence directly PS II RCs. The fraction of active PS II RCs was lowered in DF-treated cells, but energy flux per active RC increased. Our study indicates that DF phytotoxicity results mainly from photosynthesis inhibition due to “silencing” of a fraction of PS II RCs.
Autor Monika Majewska (WB / KFiBR)
Monika Majewska
- Katedra Fizjologii i Biotechnologii Roślin
, Darya Harshkova (WB / KFiBR)
Darya Harshkova
- Katedra Fizjologii i Biotechnologii Roślin
, Monika Guściora (WB)
Monika Guściora
- Wydział Biologii
, Anna Aksmann (WB / KFiBR)
Anna Aksmann
- Katedra Fizjologii i Biotechnologii Roślin
Tytuł czasopisma/seriiChemosphere, ISSN 0045-6535, (A 35 pkt)
Rok wydania2018
Tom209
Paginacja989-997
Objętość publikacji w arkuszach wydawniczych0.5
Słowa kluczowe w języku angielskimdiclofenac, atrazine, phytotoxicity, Chlamydomonas, chlorophyll fluorescence in vivo, oxidative stress
Klasyfikacja ASJC2700 General Medicine; 1600 General Chemistry; 2304 Environmental Chemistry
DOIDOI:10.1016/j.chemosphere.2018.06.156
URL https://doi.org/10.1016/j.chemosphere.2018.06.156
Języken angielski
Punktacja (całkowita)35
Żródło punktacjijournalList
PunktacjaPunktacja MNiSW = 35.0, 18-11-2019, ArticleFromJournal
Wskaźniki publikacji Cytowania WoS = 3; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.54; Impact Factor WoS: 2018 = 5.108 (2) - 2018=5.089 (5)
Liczba cytowań*7 (2019-12-06)
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* Podana liczba cytowań wynika z analizy informacji dostępnych w Internecie i jest zbliżona do wartości obliczanej przy pomocy systemu Publish or Perish.
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