Inhibition of apoptosis in BHV-1-infected cells depends on Us3 serine/threonine kinase and its enzymatic activity

Agnieszka Brzozowska , Andrea Lipińska , Natalia Derewońko , Dorota Lesiak , Michał Rychłowski , Łukasz Rąbalski , Krystyna Bieńkowska-Szewczyk


Us3 protein is a serine/threonine kinase conserved within the Alphaherpesvirinae subfamily of herpesviruses. The Us3 homologs of herpes simplex virus, pseudorabies virus, and bovine herpesvirus type 5 have been shown to block apoptosis triggered by viral infection or exogenous inducers. To determine whether these characteristics are shared by bovine herpesvirus type 1 Us3, we constructed two viral mutants: BHV-1 Us3 deletion mutant (BHV-1ΔUs3) and a kinase-dead mutant (BHV-1KD). Flow cytometry analysis and TUNEL assay clearly demonstrated, that only BHV-1 wild type virus suppressed infection-induced apoptosis and protected cells from apoptosis triggered by exogenous factors: sorbitol or staurosporine. Us3 of BHV-1 was directly capable of blocking apoptosis without the presence of other viral proteins. The presence of Us3 correlated with phosphorylation of BAD, a pro-apoptotic Bcl-2 family member. Our results clearly indicate that BHV-1 Us3 is necessary for efficient blocking of apoptosis triggered by viral infection and exogenous factors.
Author Agnieszka Brzozowska (IFB / IB / LVMB)
Agnieszka Brzozowska,,
- Laboratory of Virus Molecular Biology
, Andrea Lipińska (IFB / IB / LVMB)
Andrea Lipińska,,
- Laboratory of Virus Molecular Biology
, Natalia Derewońko (IFB)
Natalia Derewońko,,
- Intercollegiate Faculty of Biotechnology UG
, Dorota Lesiak (IFB / IB / LVMB)
Dorota Lesiak,,
- Laboratory of Virus Molecular Biology
, Michał Rychłowski (IFB / IB / LVMB)
Michał Rychłowski,,
- Laboratory of Virus Molecular Biology
, Łukasz Rąbalski (IFB / IB / LRV)
Łukasz Rąbalski,,
- Laboratory of Recombinant Vaccines
, Krystyna Bieńkowska-Szewczyk (IFB / IB / LVMB)
Krystyna Bieńkowska-Szewczyk,,
- Laboratory of Virus Molecular Biology
Journal seriesVirology, ISSN 0042-6822, (A 30 pkt)
Issue year2018
Publication size in sheets0.5
Keywords in EnglishUs3 serine/threonine kinase, alphaherpesviruses, BHV-1, apoptosis
ASJC Classification2406 Virology
Languageen angielski
Score (nominal)30
ScoreMinisterial score = 25.0, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, ArticleFromJournal
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 0.96; WoS Impact Factor: 2017 = 3.374 (2) - 2017=3.115 (5)
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