Inhibition of apoptosis in BHV-1-infected cells depends on Us3 serine/threonine kinase and its enzymatic activity
Agnieszka Brzozowska , Andrea Lipińska , Natalia Derewońko , Dorota Lesiak , Michał Rychłowski , Łukasz Rąbalski , Krystyna Bieńkowska-Szewczyk
AbstractUs3 protein is a serine/threonine kinase conserved within the Alphaherpesvirinae subfamily of herpesviruses. The Us3 homologs of herpes simplex virus, pseudorabies virus, and bovine herpesvirus type 5 have been shown to block apoptosis triggered by viral infection or exogenous inducers. To determine whether these characteristics are shared by bovine herpesvirus type 1 Us3, we constructed two viral mutants: BHV-1 Us3 deletion mutant (BHV-1ΔUs3) and a kinase-dead mutant (BHV-1KD). Flow cytometry analysis and TUNEL assay clearly demonstrated, that only BHV-1 wild type virus suppressed infection-induced apoptosis and protected cells from apoptosis triggered by exogenous factors: sorbitol or staurosporine. Us3 of BHV-1 was directly capable of blocking apoptosis without the presence of other viral proteins. The presence of Us3 correlated with phosphorylation of BAD, a pro-apoptotic Bcl-2 family member. Our results clearly indicate that BHV-1 Us3 is necessary for efficient blocking of apoptosis triggered by viral infection and exogenous factors.
|Journal series||Virology, ISSN 0042-6822, (A 30 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Us3 serine/threonine kinase, alphaherpesviruses, BHV-1, apoptosis|
|Score|| = 25.0, ArticleFromJournal|
= 30.0, ArticleFromJournal
|Publication indicators||= 0; : 2016 = 0.96; : 2017 = 3.374 (2) - 2017=3.115 (5)|
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