Design, synthesis, and enzymatic evaluation of novel ZnO quantum dot-based assay for detection of proteinase 3 activity

Jadwiga Popow-Stellmaszyk , Beata Bajorowicz , Anna Malankowska , Magdalena Wysocka , Tomasz Klimczuk , Adriana Zaleska-Medynska , Adam Lesner

Abstract

Herein, the synthesis and application of functionalized quantum dot-based protease probes is described. Such probes are composed of nontoxic ZnO nanocrystals decorated by amino groups followed by linker and labeled peptide attachment. Spherical NH2-terminated ZnO quantum dots (QDs) with the average size ranging from 4 to 8 nm and strong emission centered at 530 nm were prepared using the sol−gel method. The fluorescence of ZnO QDs was quenched by the BHQ1 moiety present on the N-terminal amino group of the peptide. The enzymatic cleavage of the peptide mediated by the proteinase 3 (PR3) bond resulted in an increase in the QD probe fluorescence. This observation was verified using both model and biological systems; and the picomolar detection limit was found to be more than 30 times lower than that of the previously reported internally quenched peptide (a decrease in detection limit from 43 to 1.3 pmol was observed).
Author Jadwiga Popow-Stellmaszyk PAB
Jadwiga Popow-Stellmaszyk,,
- Laboratory of Biochemical Analytics
, Beata Bajorowicz PFotokatalizy
Beata Bajorowicz,,
- Laboratory of Photocatalysis
, Anna Malankowska PFotokatalizy
Anna Malankowska,,
- Laboratory of Photocatalysis
, Magdalena Wysocka PChM
Magdalena Wysocka,,
- Laboratory of Medical Chemistry
, Tomasz Klimczuk
Tomasz Klimczuk,,
-
, Adriana Zaleska-Medynska PFotokatalizy
Adriana Zaleska-Medynska,,
- Laboratory of Photocatalysis
, Adam Lesner PAB
Adam Lesner,,
- Laboratory of Biochemical Analytics
Journal seriesBioconjugate Chemistry, ISSN 1043-1802
Issue year2018
Vol29
No5
Pages1576-1583
Publication size in sheets0.5
DOIDOI:10.1021/acs.bioconjchem.8b00100
URL https://pubs.acs.org/doi/10.1021/acs.bioconjchem.8b00100
Languageen angielski
Score (nominal)40
ScoreMinisterial score = 35.0, 02-05-2018, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 02-05-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 4.818 (2) - 2016=4.574 (5)
Citation count*0 (2018-08-17)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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