Design, synthesis, and enzymatic evaluation of novel ZnO quantum dot-based assay for detection of proteinase 3 activity

Jadwiga Popow-Stellmaszyk , Beata Bajorowicz , Anna Malankowska , Magdalena Wysocka , Tomasz Klimczuk , Adriana Zaleska-Medynska , Adam Lesner


Herein, the synthesis and application of functionalized quantum dot-based protease probes is described. Such probes are composed of nontoxic ZnO nanocrystals decorated by amino groups followed by linker and labeled peptide attachment. Spherical NH2-terminated ZnO quantum dots (QDs) with the average size ranging from 4 to 8 nm and strong emission centered at 530 nm were prepared using the sol−gel method. The fluorescence of ZnO QDs was quenched by the BHQ1 moiety present on the N-terminal amino group of the peptide. The enzymatic cleavage of the peptide mediated by the proteinase 3 (PR3) bond resulted in an increase in the QD probe fluorescence. This observation was verified using both model and biological systems; and the picomolar detection limit was found to be more than 30 times lower than that of the previously reported internally quenched peptide (a decrease in detection limit from 43 to 1.3 pmol was observed).
Author Jadwiga Popow-Stellmaszyk (FCh / DMB / LBA)
Jadwiga Popow-Stellmaszyk,,
- Laboratory of Biochemical Analytics
, Beata Bajorowicz (FCh / DET / LPh)
Beata Bajorowicz,,
- Laboratory of Photocatalysis
, Anna Malankowska (FCh / DET / LPh)
Anna Malankowska,,
- Laboratory of Photocatalysis
, Magdalena Wysocka (FCh / DBCh / LMCh)
Magdalena Wysocka,,
- Laboratory of Medical Chemistry
, Tomasz Klimczuk
Tomasz Klimczuk,,
, Adriana Zaleska-Medynska (FCh / DET / LPh)
Adriana Zaleska-Medynska,,
- Laboratory of Photocatalysis
, Adam Lesner (FCh / DMB / LBA)
Adam Lesner,,
- Laboratory of Biochemical Analytics
Journal seriesBioconjugate Chemistry, ISSN 1043-1802, (A 35 pkt)
Issue year2018
Publication size in sheets0.5
Languageen angielski
Score (nominal)40
ScoreMinisterial score = 35.0, 02-05-2018, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 02-05-2018, ArticleFromJournal
Publication indicators WoS Citations = 2; WoS Impact Factor: 2017 = 4.485 (2) - 2017=4.416 (5)
Citation count*2 (2019-03-23)
Share Share

Get link to the record

* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.