Short arginine-rich lipopeptides: from self-assembly to antimicrobial activity

Emilia Sikorska , Oktawian Stachurski , Damian Neubauer , Izabela Małuch , Dariusz Wyrzykowski , Marta Bauer , Krzysztof Brzozowski , Wojciech Kamysz

Abstract

In this paper, we examine antimicrobial and cytotoxic activities, self-assembly and interactions with anionic and zwitterionic membranes of short arginine-rich lipopeptides: C16-RRRR-NH2, C14-RRRR-NH2, C12-RRRR-NH2, and C16-PRRR-NH2. They show a tendency to self-assembly into micelles, but it is not required for antimicrobial activity. The membrane binding of the lipopeptides can be accompanied by other factors such as: peptide aggregation, pore formation or micellization of phospholipid bilayer. The shortening of the acyl chain results in compounds with a lower haemolytic activity and a slightly improved antimicrobial activity against Gram-positive bacteria, what indicates enhanced cell specificity. Results of coarse-grained molecular dynamics simulations indicate different organization of membrane lipids upon binding of arginine-based lipopeptides and the previously studied lysine-based ones.
Author Emilia Sikorska (FCh / DOCh / PBSB)
Emilia Sikorska,,
- Pracownia Badań Strukturalnych Biopolimerów
, Oktawian Stachurski (FCh / DOCh / PBSB)
Oktawian Stachurski,,
- Pracownia Badań Strukturalnych Biopolimerów
, Damian Neubauer
Damian Neubauer,,
-
, Izabela Małuch (FCh / DOCh / PBSB)
Izabela Małuch,,
- Pracownia Badań Strukturalnych Biopolimerów
, Dariusz Wyrzykowski (FCh / DGICh / PBChN)
Dariusz Wyrzykowski,,
- Pracownia Biologicznej Chemii Nieorganicznej
, Marta Bauer
Marta Bauer,,
-
, Krzysztof Brzozowski (FCh / NMRL)
Krzysztof Brzozowski,,
- NMR Laboratory
, Wojciech Kamysz
Wojciech Kamysz,,
-
Journal seriesBiochimica et Biophysica Acta-Biomembranes, ISSN 0005-2736, (A 35 pkt)
Issue year2018
Vol1860
No11
Pages2242-2251
Publication size in sheets0.5
Keywords in Englishlipopeptide, critical micellar concentration, self-assembly, FTIR, ITC, coarse-grained molecular dynamics
DOIDOI:10.1016/j.bbamem.2018.09.004
URL https://doi.org/10.1016/j.bbamem.2018.09.004
Languageen angielski
Score (nominal)35
ScoreMinisterial score = 35.0, 21-09-2018, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 21-09-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 3.498 (2) - 2016=3.542 (5)
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