Rapid eradication of bacterial phytopathogens by atmospheric pressure glow discharge generated in contact with a flowing liquid cathode

Agata Motyka , Anna Dzimitrowicz , Piotr Jamroz , Ewa Łojkowska , Wojciech Śledź , Pawel Pohl

Abstract

Diseases caused by phytopathogenic bacteria are responsible for significant economic losses, and these bacteria spread through diverse pathways including waterways and industrial wastes. It is therefore of high interest to develop potent methods for their eradication. Here, antibacterial properties of direct current atmospheric pressure glow discharge (dc‐APGD) generated in contact with flowing bacterial suspensions were examined against five species of phytopathogens. Complete eradication of Clavibacter michiganensis subsp. sepedonicus, Dickeya solani, and Xanthomonas campestris pv. campestris from suspensions of OD600 ≈ 0.1 was observed, while there was at least 3.43 logarithmic reduction in population densities of Pectobacterium atrosepticum and Pectobacterium carotovorum subsp. carotovorum. Analysis of plasma‐chemical parameters of the dc‐APGD system revealed its high rotational temperatures of 2,300 ± 100 K and 4,200 ± 200 K, as measured from N2 and OH molecular bands, respectively, electron temperature of 6,050 ± 400 K, vibrational temperature of 4000 ± 300 K, and high electron number density of 1.1 × 1015 cm−1. In addition, plasma treatment led to formation of numerous reactive species and states in the treated liquid, including reactive nitrogen and oxygen species such as NOx, NH, H2O2, O2, O, and OH. Further examination revealed that bactericidal activity of dc‐APGD was primarily due to presence of these reactive species as well as to UVA, UVB, and UVC irradiation generated by the dc‐APGD source. Plasma treatment also resulted in an increase in temperature (from 24.2 to 40.2 °C) and pH (from 6.0 to 10.8) of bacterial suspensions, although these changes had minor effects on cell viability. All results suggest that the newly developed dc‐APGD‐based system can be successfully implemented as a simple, rapid, efficient, and cost‐effective disinfection method for liquids originating from different industrial and agricultural settings.
Author Agata Motyka MWB UG i GUMed
Agata Motyka,,
- Intercollegiate Faculty of Biotechnology UG
, Anna Dzimitrowicz
Anna Dzimitrowicz,,
-
, Piotr Jamroz
Piotr Jamroz,,
-
, Ewa Łojkowska KB
Ewa Łojkowska,,
- Department of Biotechnology
, Wojciech Śledź KB
Wojciech Śledź,,
- Department of Biotechnology
, Pawel Pohl
Pawel Pohl,,
-
Journal seriesBiotechnology and Bioengineering, ISSN 0006-3592
Issue year2018
Vol115
No6
Pages1581-1593
Publication size in sheets0.6
Keywords in EnglishClavibacter sp., Dickeya sp., disinfection, Pectobacterium sp., plasma-liquid interactions, Xanthomonas sp.
DOIDOI:10.1002/bit.26565
URL https://doi.org/10.1002/bit.26565
Languageen angielski
Score (nominal)40
ScoreMinisterial score = 40.0, 30-05-2018, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 30-05-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 4.481 (2) - 2016=4.338 (5)
Citation count*0
Cite
Share Share

Get link to the record
msginfo.png


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back