Synthesis and evaluation of biological activity of antimicrobial: pro-proliferative peptide conjugates

Paulina Kosikowska , Michal Pikula , Paulina Langa , Piotr Trzonkowski , Michał Obuchowski , Adam Lesner

Abstract

Skin represents the largest organ of the human body and plays a crucial role in its protection from the negative impact of the outside environment, maintains its homeostasis, enables sensory interaction and thermoregulation. The traumatized skin tissue undergoes several phenotype switches due to progressive reoxygenation and release of cytokine and growth factors, that activate mechanisms of reparative processes. However, in case of wounds colonized with pathogenic microflora natural regenerative mechanisms become substantially impaired, that could lead to chronic inflammatory states with non-healing skin lesions. Herein, we present the initial results of our studies aimed at the design of bifunctional peptide-based compounds. The chemical approach, that was utilized in this work, was based on the conjugation of antimicrobial peptides with the peptides, that have potential pro-proliferative and/or cytoprotective activity towards human keratinocytes and fibroblasts, in order to obtain antimicrobials with reduced cytotoxicity or compounds that maintain both activities, i.e. inhibit bacterial or fungi growth and activate cell proliferation/migration in in vitro tests. As a result, we obtained a group of peptide conjugates that effectively inhibited the growth of selected bacterial and fungi strains and were able to stimulate proliferation and migration of keratinocytes and fibroblasts under their effective microbicidal concentrations.
Author Paulina Kosikowska (FCh / DMB / LBA)
Paulina Kosikowska,,
- Laboratory of Biochemical Analytics
, Michal Pikula
Michal Pikula,,
-
, Paulina Langa
Paulina Langa,,
-
, Piotr Trzonkowski
Piotr Trzonkowski,,
-
, Michał Obuchowski (IFB / DMedB)
Michał Obuchowski,,
- Department of Medical Biotechnology
, Adam Lesner (FCh / DMB / LBA)
Adam Lesner,,
- Laboratory of Biochemical Analytics
Journal seriesPlos One, ISSN 1932-6203, (A 35 pkt)
Issue year2015
Vol10
No10
Pages1-16
Publication size in sheets0.75
DOIDOI:10.1371/journal.pone.0140377
URL http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0140377
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)40
ScoreMinisterial score = 40.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2015 = 3.057 (2) - 2015=3.535 (5)
Citation count*7 (2018-12-14)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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