Interactions of newly synthesized platinum nanoparticles with ICR-191 and their potential application
Agnieszka Borowik , Rafał Banasiuk , Natalia Derewońko , Michał Rychłowski , Marta Krychowiak-Maśnicka , Dariusz Wyrzykowski , Magdalena Ziąbka , Anna Woziwodzka , Aleksandra Królicka , Jacek Piosik
AbstractOne of the greatest challenges of modern medicine is to find cheaper and easier ways to produce transporters for biologically active substances, which will provide selective and efficient drug delivery to the target cells, while causing low toxicity towards healthy cells. Currently, metal-based nanoparticles are considered a successful and viable solution to this problem. In this work, we propose the use of novel synthesis method of platinum nanoparticles (PtNPs) connected with their precise biophysical characterization and assessment of their potential toxicity. To work as an efficient nanodelivery platform, nanoparticles should interact with the desired active compounds spontaneously and non-covalently. We investigated possible direct interactions of PtNPs with ICR-191, a model acridine mutagen with well-established biophysical properties and mutagenic activity, by Dynamic Light Scattering, fluorescence spectroscopy, and Isothermal Titration Calorimetry. Moreover, to determine the biological activity of ICR-191-PtNPs aggregates, we employed Ames mutagenicity test, eukaryotic cell line analysis and toxicity test against the model organism Caenorhabditis elegans. PtNPs’ interesting physicochemical properties associated to the lack of toxicity in a tested range of concentrations, as well as their ability to modulate ICR-191 biological activity, suggest that these particles successfully work as potential delivery platforms for different biologically active substances.
|Journal series||Scientific Reports, ISSN 2045-2322, (N/A 140 pkt)|
|Publication size in sheets||0.5|
|License||Journal (articles only); published final; ; with publication|
|Score||= 140.0, 28-01-2020, ArticleFromJournal|
|Publication indicators||= 0; : 2016 = 1.401; : 2018 = 4.011 (2) - 2018=4.525 (5)|
|Citation count*||3 (2020-02-18)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.