Interactions of newly synthesized platinum nanoparticles with ICR-191 and their potential application

Agnieszka Borowik , Rafał Banasiuk , Natalia Derewońko , Michał Rychłowski , Marta Krychowiak-Maśnicka , Dariusz Wyrzykowski , Magdalena Ziąbka , Anna Woziwodzka , Aleksandra Królicka , Jacek Piosik

Abstract

One of the greatest challenges of modern medicine is to find cheaper and easier ways to produce transporters for biologically active substances, which will provide selective and efficient drug delivery to the target cells, while causing low toxicity towards healthy cells. Currently, metal-based nanoparticles are considered a successful and viable solution to this problem. In this work, we propose the use of novel synthesis method of platinum nanoparticles (PtNPs) connected with their precise biophysical characterization and assessment of their potential toxicity. To work as an efficient nanodelivery platform, nanoparticles should interact with the desired active compounds spontaneously and non-covalently. We investigated possible direct interactions of PtNPs with ICR-191, a model acridine mutagen with well-established biophysical properties and mutagenic activity, by Dynamic Light Scattering, fluorescence spectroscopy, and Isothermal Titration Calorimetry. Moreover, to determine the biological activity of ICR-191-PtNPs aggregates, we employed Ames mutagenicity test, eukaryotic cell line analysis and toxicity test against the model organism Caenorhabditis elegans. PtNPs’ interesting physicochemical properties associated to the lack of toxicity in a tested range of concentrations, as well as their ability to modulate ICR-191 biological activity, suggest that these particles successfully work as potential delivery platforms for different biologically active substances.
Author Agnieszka Borowik (IFB)
Agnieszka Borowik,,
- Intercollegiate Faculty of Biotechnology UG
, Rafał Banasiuk (IFB / IB / DBiotech)
Rafał Banasiuk,,
- Department of Biotechnology
, Natalia Derewońko (IFB)
Natalia Derewońko,,
- Intercollegiate Faculty of Biotechnology UG
, Michał Rychłowski (IFB / IB / LVMB)
Michał Rychłowski,,
- Laboratory of Virus Molecular Biology
, Marta Krychowiak-Maśnicka (IFB / IB / DBiotech)
Marta Krychowiak-Maśnicka,,
- Department of Biotechnology
, Dariusz Wyrzykowski (FCh / DGICh / PBChN)
Dariusz Wyrzykowski,,
- Pracownia Biologicznej Chemii Nieorganicznej
, Magdalena Ziąbka
Magdalena Ziąbka,,
-
, Anna Woziwodzka (IFB / M020 / DMCB)
Anna Woziwodzka,,
- Department of Molecular and Cellular Biology
, Aleksandra Królicka (IFB / IB / DBiotech)
Aleksandra Królicka,,
- Department of Biotechnology
, Jacek Piosik (IFB / M020 / DMCB)
Jacek Piosik,,
- Department of Molecular and Cellular Biology
Journal seriesScientific Reports, ISSN 2045-2322, (A 40 pkt)
Issue year2019
Vol9
No1
Pages1-11
Publication size in sheets0.5
ASJC Classification1000 Multidisciplinary
DOIDOI:10.1038/s41598-019-41092-6
URL https://rdcu.be/bCZ13
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)40
ScoreMinisterial score = 40.0, 24-07-2019, ArticleFromJournal
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.401; WoS Impact Factor: 2017 = 4.122 (2) - 2017=4.609 (5)
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