Secondary metabolites in in vitro cultured plants of the genus Drosera

Ł. Marczak , Anna Kawiak , Ewa Łojkowska , Mackis Stobiecki

Abstract

Extracts from plantlets of different species of the genus Drosera, grown as in vitro cultures, were evaluated for the level of phenolic secondary metabolites from the group of naphthoquinones and flavonols. The profiles of natural products in the extracts obtained from different species were monitored by HPLC with UV detection at 260 and 330 nm. On the basis of the data obtained, Drosera binata, the species with the highest amount of plumbagin, was selected for further studies. The most effective method of extraction of quinones was established and the composition of phenolic secondary metabolites in the tissues was determined. For the identification of phenolic compounds, HPLC-UV and HPLC-ESI/MS were applied.
Author Ł. Marczak - [Instytut Chemii Bioorganicznej Polskiej Akademii Nauk]
Ł. Marczak,,
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, Anna Kawiak (IFB / IB / DBiotech)
Anna Kawiak,,
- Department of Biotechnology
, Ewa Łojkowska (IFB / IB / DBiotech)
Ewa Łojkowska,,
- Department of Biotechnology
, Mackis Stobiecki - [Instytut Chemii Bioorganicznej Polskiej Akademii Nauk]
Mackis Stobiecki,,
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Journal seriesPhytochemical Analysis, ISSN 0958-0344, e-ISSN 1099-1565, (A 25 pkt)
Issue year2005
Vol16
No3
Pages143-149
ASJC Classification2700 General Medicine; 2707 Complementary and alternative medicine; 1106 Food Science; 1110 Plant Science; 1303 Biochemistry; 1313 Molecular Medicine; 3002 Drug Discovery; 1602 Analytical Chemistry
DOIDOI:10.1002/pca.833
URL https://doi.org/10.1002/pca.833
Languageen angielski
Score (nominal)25
Score sourcejournalList
Publication indicators Scopus Citations = 36; Scopus SNIP (Source Normalised Impact per Paper): 2005 = 1.005; WoS Impact Factor: 2006 = 1.228 (2) - 2007=1.812 (5)
Citation count*52 (2020-01-16)
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