Female gametophyte development in Sedum sediforme (Jacq.) Pau (Crassulaceae): an anatomical, cytochemical and ultrastructural analysis

Emilia Brzezicka , Małgorzata Kozieradzka-Kiszkurno

Abstract

Available documentation about the development of the female gametophyte of Crassulaceae is very limited. The aim of this study was to extend the embryological knowledge of Crassulaceae by analysing the development of the embryo sac in Sedum sediforme. Transmission electron microscopy and light microscopy including Nomarski optics (DIC) were used to observe individual stages of female gametophyte development. Cytochemical staining enabled detection of lipids, insoluble polysaccharides and proteins in gametophyte cells during their formation. Their increased accumulation was observed during nucellar cell and unfunctional cell degeneration in the embryo sac at the coenocytic and cellular stages (megagametogenesis). The female gametophyte develops in anatropous, bitegmic and crassinucellate ovules. The mature embryo sac is built of seven cells but after antipodes degeneration it is formed by the egg apparatus and a central cell. The monosporic Polygonum type was observed. One megaspore mother cell (MMC) formed three cells after meiosis. A triad was formed from a functional megaspore (placed chalazally), one uninucleate megaspore and a binucleate cell located at the micropylar end. Plasmodesmata with adhering electron-dense dome were noticed in walls of the coenocytic embryo sac and in the outer walls of ephemeral antipodes. Moreover, similar to synergids, antipodes form wall ingrowths. Here, we report new structural features of the antipodal cells (the presence of plasmodesmata with an electron-dense dome) which have not been described before. This new structural observation indicates that these cells participate in substance transport and that this process can probably be additionally regulated.
Author Emilia Brzezicka (FB / DPCE)
Emilia Brzezicka ,,
- Department of Plant Cytology and Embryology
, Małgorzata Kozieradzka-Kiszkurno (FB / DPCE)
Małgorzata Kozieradzka-Kiszkurno,,
- Department of Plant Cytology and Embryology
Journal seriesProtoplasma, ISSN 0033-183X, (A 30 pkt)
Issue year2019
Vol256
No2
Pages537-553
Publication size in sheets0.8
Keywords in Englishantipodal cells, embryo sac, megagametogenesis, megasporogenesis, plasmodesmata, wall ingrowths
ASJC Classification1307 Cell Biology; 1110 Plant Science; 2700 General Medicine
DOIDOI:10.1007/s00709-018-1319-9
URL https://link.springer.com/content/pdf/10.1007%2Fs00709-018-1319-9.pdf
Languageen angielski
LicenseOther; published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)30
ScoreMinisterial score = 30.0, 24-07-2019, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2016 = 0.962; WoS Impact Factor: 2017 = 2.457 (2) - 2017=2.43 (5)
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