Elicitation strategies for the improvement of essential oil content in Rhododendron tomentosum (Ledum palustre) bioreactor-grown microshoots

Anna Jesionek , Adam Kokotkiewicz , Aleksandra Królicka , Bożena Zabiegała , Maria Luczkiewicz

Abstract

Rhododendron tomentosum Harmaja, previously Ledum palustre L., is a fragrant plant growing in swampy areas, which is characterized by well-known repellent properties and the anti-arthritic potential. In the present study, R. tomentosum microshoots cultivated in RITA® bioreactor, providing the alternative, environment-independent source of the valuable essential oil, were treated for 7 days with copper and nickel salts, methyl jasmonate, chitosan, ergosterol, lysates of: Candida albicans, Escherichia coli, Enterobacter sakazaki, Pectobacterium carotovorum and Dickeya dadantii, to stimulate the production of secondary metabolites. Moreover, for the first time, the aphid ethanol extract was tested as an elicitor of insect origin. Suspensions of all pathogens added to in vitro culture reduced significantly the biomass growth parameters. The application of the aphid extract and lysate of P. carotovorum increased the volatile fraction content by 14%, while Ni2+ and ergosterol by 8%. The influence of the studied elicitors on the chemical composition of R. tomentosum essential oils was presented and discussed thoroughly, taking into account a percentage of 22 main terpenoid compounds, determined by GC/MS (i.a. p-cymene, geranyl acetate, δ-cadinene, shyobunone, methyl everninate, ledene oxide (II) and alloaromadendrene). The biosynthesis of monoterpenes was primarily proven to be affected by the elicitation. The conducted experiments suggest that R. tomentosum is rather resistant to elicitation strategies, both abiotic and biotic as well as methyl jasmonate.
Author Anna Jesionek
Anna Jesionek,,
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, Adam Kokotkiewicz
Adam Kokotkiewicz,,
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, Aleksandra Królicka (IFB / IB / DBiotech)
Aleksandra Królicka,,
- Department of Biotechnology
, Bożena Zabiegała
Bożena Zabiegała,,
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, Maria Luczkiewicz
Maria Luczkiewicz,,
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Journal seriesIndustrial Crops and Products, ISSN 0926-6690, (A 40 pkt)
Issue year2018
Vol123
Pages461-469
Publication size in sheets0.5
Keywords in Englishabiotic and biotic elicitation, GC/MS analysis, insect-induced stress, in vitro cultures, RITA Bioreactor, terpenes and terpenoids
DOIDOI:10.1016/j.indcrop.2018.07.013
URL https://doi.org/10.1016/j.indcrop.2018.07.013
Languageen angielski
Score (nominal)40
ScoreMinisterial score = 40.0, 26-10-2018, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 26-10-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 3.181 (2) - 2016=3.577 (5)
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