Relationship between copper(II) complexes with FomA adhesin fragments of F.nucleatum and colorectal cancer. Coordination pattern and ability to promote ROS production

M. K. Lesiów , U. K. Komarnicka , K. Stokowa-Sołtys , Krzysztof Rolka , Anna Łęgowska , Natalia Ptaszyńska , R. Wieczorek , A. Kyzioł , M. Jeżowska-Bojczuk

Abstract

The copper(II) ion binding of the Ac-KGHGNG-NH2 and Ac-PTVHNE-NH2 fragments of FomA adhesin from Fusobacterium nucleatum was studied using potentiometry, UV-Vis, CD, EPR and DFT techniques. The coordination pattern was described in a wide range of pH values. Ligands begin interactions with metal ions using imidazole nitrogen. At pH 6.8 (a value typical of the large intestine environment), the metal ion was coordinated by the 3N donor atoms {Nim, 2 × N−amide} in both cases. However, the copper(II) ion was bound more effectively by the Ac-PTVHNE-NH2 peptide. The formation of reactive oxygen species (ROS) was studied by UV-Vis and fluorescence spectroscopy, as well as gel electrophoresis in the presence of H2O2 and/or ascorbic acid. The complexes generated ROS in the highest amounts among all compounds. Moreover, they stimulated the CT26 cell line (mouse colon carcinoma) to produce ROS which lead to oxidative stress. It was also determined that such radicals took part in the plasmid degradation mechanism.
Author M. K. Lesiów
M. K. Lesiów,,
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, U. K. Komarnicka
U. K. Komarnicka,,
-
, K. Stokowa-Sołtys
K. Stokowa-Sołtys,,
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, Krzysztof Rolka (FCh / DMB / LBCh)
Krzysztof Rolka,,
- Laboratory of Bioorganic Chemistry
, Anna Łęgowska (FCh / DMB / LBCh)
Anna Łęgowska,,
- Laboratory of Bioorganic Chemistry
, Natalia Ptaszyńska (FCh / DMB / LBCh)
Natalia Ptaszyńska,,
- Laboratory of Bioorganic Chemistry
, R. Wieczorek
R. Wieczorek,,
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, A. Kyzioł
A. Kyzioł,,
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, M. Jeżowska-Bojczuk
M. Jeżowska-Bojczuk,,
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Journal seriesDalton Transactions: the international journal for inorganic, organometallic and bioinorganic chemistry , ISSN 1477-9226, (A 40 pkt)
Issue year2018
Vol47
No15
Pages5445-5458
Publication size in sheets0.65
Keywords in Englishcomplexes of copper(II), FomA, F. nucleatum, ROS, potentiometry
DOIDOI:10.1039/c7dt04103a
URL http://pubs.rsc.org/en/content/articlepdf/2018/dt/c7dt04103a
Languageen angielski
Score (nominal)40
ScoreMinisterial score = 40.0, 02-07-2018, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 02-07-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 4.029 (2) - 2016=3.851 (5)
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