Trehalose protects Escherichia coli against carbon stress manifested by protein acetylation and aggregation

Maria Moruno Algara , Dorota Kuczyńska-Wiśnik , Janusz Dębski , Karolina Stojowska-Swędrzyńska , Hanna Sominka , Małgorzata Bukrejewska , Ewa Laskowska

Abstract

The disaccharide trehalose is widely distributed in nature and can serve as a carbon reservoir, a signaling molecule for controlling glucose metabolism and a stress protectant. We demonstrated that in Escherichia coliΔotsA cells, which are unable to synthesize trehalose, the aggregation of endog-enous proteins during the stationary phase was increased in comparison to wild-type cells. The lack of trehalose synthesis boosted Nε-lysine acetylation of proteins, which in turn enhanced their hydrophobicity and aggregation. This increased Nε-lysine acetylation could result from carbon overflow and the accumulation of acetyl phosphate caused by the ΔotsA mutation. These findings provide a better understanding of the previously reported protective functions of trehalose in protein stabilization and the prevention of protein aggregation. Our results indicate that trehalose may participate in proteosta-sis not only as a chemical chaperone but also as a metabolite that indirectly counteracts detrimental protein acetylation. We propose that trehalose protects E. coli against carbon stress – the synthesis and storage of trehalose can prevent carbon overflow, which otherwise is manifested by protein acetylation and aggregation.
Publication typeIn press (online first, early view)
Author Maria Moruno Algara (FB / DB)
Maria Moruno Algara,,
- Department of Biochemistry
, Dorota Kuczyńska-Wiśnik (FB / DB)
Dorota Kuczyńska-Wiśnik,,
- Department of Biochemistry
, Janusz Dębski
Janusz Dębski,,
-
, Karolina Stojowska-Swędrzyńska (FB / DB)
Karolina Stojowska-Swędrzyńska,,
- Department of Biochemistry
, Hanna Sominka (FB / DMBG)
Hanna Sominka,,
- Katedra Biologii i Genetyki Medycznej
, Małgorzata Bukrejewska (FB / DB)
Małgorzata Bukrejewska,,
- Department of Biochemistry
, Ewa Laskowska (FB / DB)
Ewa Laskowska,,
- Department of Biochemistry
Journal seriesMolecular Microbiology, ISSN 0950-382X, (A 35 pkt)
Issue year2019
Noonline first
Pages1-15
Publication size in sheets0.7
ASJC Classification1312 Molecular Biology; 2404 Microbiology
DOIDOI:10.1111/mmi.14322
URL https://doi.org/10.1111/mmi.14322
Languageen angielski
Score (nominal)35
ScoreMinisterial score = 35.0, 06-08-2019, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.043; WoS Impact Factor: 2017 = 3.816 (2) - 2017=4.032 (5)
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