Trehalose protects Escherichia coli against carbon stress manifested by protein acetylation and aggregation

Maria Moruno Algara , Dorota Kuczyńska-Wiśnik , Janusz Dębski , Karolina Stojowska-Swędrzyńska , Hanna Sominka , Małgorzata Bukrejewska , Ewa Laskowska

Abstract

The disaccharide trehalose is widely distributed in nature and can serve as a carbon reservoir, a signaling molecule for controlling glucose metabolism and a stress protectant. We demonstrated that in Escherichia coliΔotsA cells, which are unable to synthesize trehalose, the aggregation of endog-enous proteins during the stationary phase was increased in comparison to wild-type cells. The lack of trehalose synthesis boosted Nε-lysine acetylation of proteins, which in turn enhanced their hydrophobicity and aggregation. This increased Nε-lysine acetylation could result from carbon overflow and the accumulation of acetyl phosphate caused by the ΔotsA mutation. These findings provide a better understanding of the previously reported protective functions of trehalose in protein stabilization and the prevention of protein aggregation. Our results indicate that trehalose may participate in proteosta-sis not only as a chemical chaperone but also as a metabolite that indirectly counteracts detrimental protein acetylation. We propose that trehalose protects E. coli against carbon stress – the synthesis and storage of trehalose can prevent carbon overflow, which otherwise is manifested by protein acetylation and aggregation.
Author Maria Moruno Algara (FB / DB)
Maria Moruno Algara,,
- Department of Biochemistry
, Dorota Kuczyńska-Wiśnik (FB / DB)
Dorota Kuczyńska-Wiśnik,,
- Department of Biochemistry
, Janusz Dębski
Janusz Dębski,,
-
, Karolina Stojowska-Swędrzyńska (FB / DB)
Karolina Stojowska-Swędrzyńska,,
- Department of Biochemistry
, Hanna Sominka (FB / DMBG)
Hanna Sominka,,
- Katedra Biologii i Genetyki Medycznej
, Małgorzata Bukrejewska (FB / DB)
Małgorzata Bukrejewska,,
- Department of Biochemistry
, Ewa Laskowska (FB / DB)
Ewa Laskowska,,
- Department of Biochemistry
Journal seriesMolecular Microbiology, ISSN 0950-382X, (N/A 100 pkt)
Issue year2019
Vol112
No3
Pages866-880
Publication size in sheets0.7
ASJC Classification1312 Molecular Biology; 2404 Microbiology
DOIDOI:10.1111/mmi.14322
URL https://doi.org/10.1111/mmi.14322
Languageen angielski
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 17-11-2019, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.043; WoS Impact Factor: 2018 = 3.649 (2) - 2018=3.819 (5)
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