Antimicrobial activity of chimera peptides composed of human neutrophil peptide 1 (HNP-1) truncated analogues and bovine lactoferrampin

Natalia Ptaszyńska , Katarzyna Gucwa , Anna Łęgowska , Dawid Dębowski , Agata Gitlin-Domagalska , Jan Lica , Mateusz Heldt , Dorota Martynow , Mateusz Olszewski , Sławomir Milewski , Tzi Bun Ng , Krzysztof Rolka

Abstract

Three chimera peptides composed of bovine lactoferrampin and the analogue of truncated human neutrophil peptide 1 were synthesized by the solid-phase method. In two compounds peptide chains were connected via isopeptide bond, whereas in the third one disulfide bridge served as a linker. All three chimeras displayed significantly higher antimicrobial activity than the constituent peptides as well as their equimolar mixtures. The one with a disulfide bridge displayed selectivity toward Gram-positive bacteria and was able to penetrate bacterial cells. The chimeric peptides demonstrated low in vitro mammalian cytotoxicity, especially against benign cells. The significance of linker type was also reflected in the secondary structure and proteolytic stability of studied compounds. Presented results proved that such chimeras are good lead structures for designing antimicrobial drugs.
Publication typeIn press (online first, early view)
Author Natalia Ptaszyńska (FCh / DMB / LBCh)
Natalia Ptaszyńska,,
- Laboratory of Bioorganic Chemistry
, Katarzyna Gucwa
Katarzyna Gucwa,,
-
, Anna Łęgowska (FCh / DMB / LBCh)
Anna Łęgowska,,
- Laboratory of Bioorganic Chemistry
, Dawid Dębowski (FCh / DMB / LBCh)
Dawid Dębowski,,
- Laboratory of Bioorganic Chemistry
, Agata Gitlin-Domagalska (FCh / DMB / LBCh)
Agata Gitlin-Domagalska,,
- Laboratory of Bioorganic Chemistry
, Jan Lica
Jan Lica,,
-
, Mateusz Heldt
Mateusz Heldt,,
-
, Dorota Martynow
Dorota Martynow,,
-
, Mateusz Olszewski
Mateusz Olszewski,,
-
, Sławomir Milewski
Sławomir Milewski,,
-
et al.`
Journal seriesBioconjugate Chemistry, ISSN 1043-1802, (A 35 pkt)
Issue year2018
Noonline first
Pages1-12
Publication size in sheets0.55
DOIDOI:10.1021/acs.bioconjchem.8b00440
URL https://pubs.acs.org/doi/10.1021/acs.bioconjchem.8b00440
Languageen angielski
Score (nominal)40
ScoreMinisterial score = 35.0, 10-09-2018, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 10-09-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 4.818 (2) - 2016=4.574 (5)
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