Identification of bacterial species in probiotic consortiums in selected commercial cleaning preparations

Joanna Jeżewska-Frąckowiak , Joanna Żebrowska , Edyta Czajkowska , Jolanta Jasińska , Monika Pęksa , Gabriela Jędrzejczak , Piotr Skowron

Abstract

The role of environmentally coexisting microflora that often comprises human commensal microbiome is still underestimated. Modern lifestyle changes include hygienic practices, food preparation and eradication of many contagious diseases. In this context, probiotic microorganisms are biocontrol remedies still under development, solving a number of gastrointestinal and immunological issues, while fighting hazardous microbiological biofilms on different surfaces. Probiotics are mainly associated with Lactic Acid Bacteria, however environmental, non-dairy sources are promising ecological niches of probiotic spore-forming Bacillus species. Industrial applications of these “unconventional” probiotics take an advantage of their sporulating activity which greatly enhances their compatibility with chemical formulations used in the household, cosmetic or pharmaceutical chemistry. We have analysed 14 commercially available chemical products, labelled or described to contain a probiotic or biologically active component. It was determined that in the most part they relay on consortiums of spore-forming, very closely related Bacillus species, exhibiting bimodal existence in the environment and the gastrointestinal tract (GIT). In addition, we have found a number of non-sporulating species. Overall, the microorganisms found included: Bacillus licheniformis, Bacillus subtilis, Bacillus pumilus, Citrobacter freundii, Klebsiella oxytoca, Stenotrophomonas malthophila, Serratia liquefaciens, Bacillus altitudinis, Lactobacillus gastricus, Bacillus megaterium, Lactobacillus nagelii, Aromatoleum buckelii, Trichosporon mucoides, Clostridium novyi, Bacteroides uniformis. As some of the listed species may become opportunistic pathogens, this raises an important question concerning general safety of probiotics, as apparently the manufacturing procedures do not always lead to microbiologically defined or sufficiently controlled microorganism consortiums.
Author Joanna Jeżewska-Frąckowiak (FCh / DMBt / LGE)
Joanna Jeżewska-Frąckowiak,,
- Laboratory of Genetic Engineering
, Joanna Żebrowska (FCh / DMBt / LGE)
Joanna Żebrowska,,
- Laboratory of Genetic Engineering
, Edyta Czajkowska (FCh / DMBt / LGE)
Edyta Czajkowska ,,
- Laboratory of Genetic Engineering
, Jolanta Jasińska
Jolanta Jasińska,,
-
, Monika Pęksa
Monika Pęksa,,
-
, Gabriela Jędrzejczak
Gabriela Jędrzejczak,,
-
, Piotr Skowron (FCh / DMBt / LGE)
Piotr Skowron,,
- Laboratory of Genetic Engineering
Journal seriesActa Biochimica Polonica, ISSN 0001-527X, (N/A 40 pkt)
Issue year2019
Vol66
No2
Pages215-222
Publication size in sheets0.5
Keywords in EnglishBacillus sp. for detergents, Bacillus sp. probiotic identification, Bacillus sp. probiotic safety, Bacillus subtilis, probiotic consortium, probiotic formulations, spore formers
ASJC Classification1300 General Biochemistry, Genetics and Molecular Biology
DOIDOI:10.18388/abp.2018_2782
URL https://doi.org/10.18388/abp.2018_2782
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa - Na Tych Samych Warunkach (CC-BY-SA); with publication
Score (nominal)40
Score sourcejournalList
ScoreMinisterial score = 40.0, 17-11-2019, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.64; WoS Impact Factor: 2018 = 1.626 (2) - 2018=1.522 (5)
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