Immunosensor based on long-period fiber gratings for detection of viruses causing gastroenteritis

Marta Janczuk-Richter , Beata Gromadzka , Łukasz Richter , Mirosława Panasiuk , Karolina Zimmer , Predrag Mikulic , Wojtek J. Bock , Sebastian Maćkowski , Mateusz Śmietana , Joanna Niedziółka Jönsson

Abstract

Since the norovirus is the main cause of acute gastroenteritis all over the world, its fast detection is crucial in medical diagnostics. In this work, a rapid, sensitive, and selective optical fiber biosensor for the detection of norovirus virus-like particles (VLPs) is reported. The sensor is based on highly sensitive long-period fiber gratings (LPFGs) coated with antibodies against the main coat protein of the norovirus. Several modification methods were verified to obtain reliable immobilization of protein receptors on the LPFG surface. We were able to detect 1 ng/mL norovirus VLPs in a 40-min assay in a label-free manner. Thanks to the application of an optical fiber as the sensor, there is a possibility to increase the user’s safety by separating the measurement point from the signal processing setup. Moreover, our sensor is small and light, and the proposed assay is straightforward. The designed LPFG-based biosensor could be applied in both fast norovirus detection and in vaccine testing.
Author Marta Janczuk-Richter
Marta Janczuk-Richter,,
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, Beata Gromadzka (IFB / IB / LRV)
Beata Gromadzka,,
- Laboratory of Recombinant Vaccines
, Łukasz Richter
Łukasz Richter,,
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, Mirosława Panasiuk (IFB)
Mirosława Panasiuk,,
- Intercollegiate Faculty of Biotechnology UG
, Karolina Zimmer (IFB)
Karolina Zimmer,,
- Intercollegiate Faculty of Biotechnology UG
, Predrag Mikulic
Predrag Mikulic,,
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, Wojtek J. Bock
Wojtek J. Bock,,
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, Sebastian Maćkowski
Sebastian Maćkowski,,
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, Mateusz Śmietana
Mateusz Śmietana,,
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, Joanna Niedziółka Jönsson
Joanna Niedziółka Jönsson,,
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Journal seriesSensors, [SENSORS-BASEL], ISSN 1424-8220, e-ISSN 1424-3210, (N/A 100 pkt)
Issue year2020
Vol20
No3
Pages1-11
Publication size in sheets0.5
Article number813
Keywords in Englishvirus detection; norovirus, virus-like particle, label-free biosensor, optical fiber sensor, long-period fiber gratings
ASJC Classification1303 Biochemistry; 1602 Analytical Chemistry; 2208 Electrical and Electronic Engineering; 3107 Atomic and Molecular Physics, and Optics
DOIDOI:10.3390/s20030813
URL https://doi.org/10.3390/s20030813
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 09-03-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.393; WoS Impact Factor: 2018 = 3.031 (2) - 2018=3.302 (5)
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