Roles of orf60a and orf61 in development of bacteriophages λ and Φ24B
Aleksandra Dydecka , Bożena Nejman-Faleńczyk , Sylwia Bloch , Gracja Topka , Agnieszka Necel , Logan Donaldson , Grzegorz Węgrzyn , Alicja Węgrzyn
AbstractThe exo-xis region of lambdoid bacteriophage genomes contains several established and potential genes that are evolutionarily conserved, but not essential for phage propagation under laboratory conditions. Nevertheless, deletion or overexpression of either the whole exo-xis region and important regulatory elements can significantly influence the regulation of phage development. This report defines specific roles for orf60a and orf61 in bacteriophage λ and Φ24B, a specific Shiga toxin-converting phage with clinical relevance. We observed that mutant phages bearing deletions of orf60a and orf61 impaired two central aspects of phage development: the lysis-versus-lysogenization decision and prophage induction. These effects were more pronounced for phage Φ24B than for λ. Surprisingly, adsorption of phage Φ24B on Escherichia coli host cells was less efficient in the absence of either orf60a or orf61. We conclude that these open reading frames (ORFs) play important, but not essential, roles in the regulation of lambdoid phage development. Although phages can propagate without these ORFs in nutrient media, we suggest that they may be involved in the regulatory network, ensuring optimization of phage development under various environmental conditions.
|Journal series||Viruses-Basel, ISSN 1999-4915, (A 30 pkt)|
|Publication size in sheets||0.6|
|Keywords in English||lambdoid phages, Shiga toxin-converting bacteriophage, regulation of bacteriophage development, exo-xis region|
|License||Journal (articles only); published final; ; with publication|
|Score||= 30.0, 20-03-2020, ArticleFromJournal|
|Publication indicators||= 1; = 1; : 2016 = 1.018; : 2018 = 3.811 (2) - 2018=3.916 (5)|
|Citation count*||1 (2020-03-30)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.