Roles of orf60a and orf61 in development of bacteriophages λ and Φ24B

Aleksandra Dydecka , Bożena Nejman-Faleńczyk , Sylwia Bloch , Gracja Topka , Agnieszka Necel , Logan Donaldson , Grzegorz Węgrzyn , Alicja Węgrzyn

Abstract

The exo-xis region of lambdoid bacteriophage genomes contains several established and potential genes that are evolutionarily conserved, but not essential for phage propagation under laboratory conditions. Nevertheless, deletion or overexpression of either the whole exo-xis region and important regulatory elements can significantly influence the regulation of phage development. This report defines specific roles for orf60a and orf61 in bacteriophage λ and Φ24B, a specific Shiga toxin-converting phage with clinical relevance. We observed that mutant phages bearing deletions of orf60a and orf61 impaired two central aspects of phage development: the lysis-versus-lysogenization decision and prophage induction. These effects were more pronounced for phage Φ24B than for λ. Surprisingly, adsorption of phage Φ24B on Escherichia coli host cells was less efficient in the absence of either orf60a or orf61. We conclude that these open reading frames (ORFs) play important, but not essential, roles in the regulation of lambdoid phage development. Although phages can propagate without these ORFs in nutrient media, we suggest that they may be involved in the regulatory network, ensuring optimization of phage development under various environmental conditions.
Author Aleksandra Dydecka (FB / DMoB)
Aleksandra Dydecka ,,
- Department of Molecular Biology
, Bożena Nejman-Faleńczyk (FB / DMoB)
Bożena Nejman-Faleńczyk,,
- Department of Molecular Biology
, Sylwia Bloch (FB / DMoB)
Sylwia Bloch,,
- Department of Molecular Biology
, Gracja Topka (FB / DMoB)
Gracja Topka,,
- Department of Molecular Biology
, Agnieszka Necel (FB / DMoB)
Agnieszka Necel,,
- Department of Molecular Biology
, Logan Donaldson
Logan Donaldson,,
-
, Grzegorz Węgrzyn (FB / DMoB)
Grzegorz Węgrzyn,,
- Department of Molecular Biology
, Alicja Węgrzyn
Alicja Węgrzyn,,
-
Journal seriesViruses-Basel, ISSN 1999-4915, (A 30 pkt)
Issue year2018
Vol10
No10
Pages1-13
Publication size in sheets0.6
Keywords in Englishlambdoid phages, Shiga toxin-converting bacteriophage, regulation of bacteriophage development, exo-xis region
DOIDOI:10.3390/v10100553
URL https://doi.org/10.3390/v10100553
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)30
ScoreMinisterial score = 30.0, 07-11-2018, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 07-11-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 3.465 (2) - 2016=3.64 (5)
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