Flavonol-based fluorescent indicator for determination of β-glucosidase activity

Illia Serdiuk , Milena Reszka , Henryk Myszka , Karol Krzymiński , Beata Liberek , Alexander D. Roshal

Abstract

A novel fluorescent probe, based on the excited-state intramolecular proton transfer (ESIPT) phenomenon, for evaluation of β-glucosidase activity was designed. The synthesis of the probe was optimized. The conditions for the monitoring of enzymatic cleavage of the probe were developed and discussed from the point of view of reaction kinetics and simplicity of the method.
Author Illia Serdiuk (FCh / DPCh / PK)
Illia Serdiuk,,
- Pracownia Krystalochemii
, Milena Reszka (FCh / DOCh / LG)
Milena Reszka,,
- Laboratory of Glycochemistry
, Henryk Myszka (FCh / DOCh / LG)
Henryk Myszka,,
- Laboratory of Glycochemistry
, Karol Krzymiński (FCh / DPCh / LLR)
Karol Krzymiński,,
- Laboratory of Luminescence Research
, Beata Liberek (FCh / DOCh / LG)
Beata Liberek,,
- Laboratory of Glycochemistry
, Alexander D. Roshal
Alexander D. Roshal,,
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Journal seriesRSC Advances, ISSN 2046-2069, (A 30 pkt)
Issue year2016
Vol6
No48
Pages42532-42536
Publication size in sheets0.5
DOIDOI:10.1039/c6ra06062e
URL http://pubs.rsc.org/en/content/articlepdf/2016/ra/c6ra06062e
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa - Użycie Niekomercyjne (CC-BY-NC); with publication
Score (nominal)35
ScoreMinisterial score = 30.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 3.108 (2) - 2016=3.257 (5)
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