Flavonol-based fluorescent indicator for determination of β-glucosidase activity
Illia Serdiuk , Milena Reszka , Henryk Myszka , Karol Krzymiński , Beata Liberek , Alexander D. Roshal
AbstractA novel fluorescent probe, based on the excited-state intramolecular proton transfer (ESIPT) phenomenon, for evaluation of β-glucosidase activity was designed. The synthesis of the probe was optimized. The conditions for the monitoring of enzymatic cleavage of the probe were developed and discussed from the point of view of reaction kinetics and simplicity of the method.
|Journal series||RSC Advances, ISSN 2046-2069, (A 30 pkt)|
|Publication size in sheets||0.5|
|License||Journal (articles only); published final; ; with publication|
|Score|| = 30.0, 20-12-2017, ArticleFromJournal|
= 35.0, 20-12-2017, ArticleFromJournal
|Publication indicators||: 2016 = 3.108 (2) - 2016=3.257 (5)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.