Overall conformation of covalently stabilized domain-swapped dimer of human cystatin C in solution

Magdalena Murawska , Aneta Szymańska , Anders Grubb , Maciej Kozak

Abstract

Human cystatin C (HCC), a small protein, plays a crucial role in inhibition of cysteine proteases. The most common structural form of human cystatin C in crystals is a dimer, which has been evidenced both for the native protein and its mutants. In these structures, HCC dimers were formed through the mechanism of domain swapping. The structure of the monomeric form of human cystatin C was determined for V57N mutant and the mutant with the engineered disulfide bond (L47C)–(G69C) (known as stab1-HCC). On the basis of stab1-HCC, a number of covalently stabilized oligomers, including also dimers have been obtained. The aim of this study was to analyze the structure of the covalently stabilized dimer HCC in solution by the small angle X-ray scattering (SAXS) technique and synchrotron radiation. Experimental data confirmed that in solution this protein forms a dimer, which is characterized by the radius of gyration RG = 3.1 nm and maximum intramolecular distance Dmax = 10.3 nm. Using the ab initio method and program DAMMIN, we propose a low resolution structure of stabilized covalently cystatin C in solution. Stab-HCC dimer adopts in solution an elongated conformation, which is well reconstructed by the ab initio model.
Author Magdalena Murawska
Magdalena Murawska,,
-
, Aneta Szymańska PChM
Aneta Szymańska,,
- Laboratory of Medical Chemistry
, Anders Grubb
Anders Grubb,,
-
, Maciej Kozak
Maciej Kozak,,
-
Journal seriesNuclear Instruments & Methods in Physics Research. Section B: Beam Interactions With Materials and Atoms, ISSN 0168-583X
Issue year2017
Vol411
Pages136-140
Publication size in sheets0.5
Keywords in Englishhuman cystatin C, shape determination, small angle X-ray scattering, synchrotron radiation
DOIDOI:10.1016/j.nimb.2017.02.090
URL https://doi.org/10.1016/j.nimb.2017.02.090
Languageen angielski
Score (nominal)25
ScoreMinisterial score = 25.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 25.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 1.109 (2) - 2016=1.113 (5)
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