Cyclopropaneoctanoic acid 2-hexyl upregulates the expression of genes responsible for lipid synthesis and release in human hepatic HepG2 cells

Aleksandra Czumaj , Adriana Mika , Michał Chmielewski , Tomasz Sledzinski

Abstract

Recently we have found cyclopropaneoctanoic acid 2‐hexyl (CPOA2H) in humans and demonstrated its elevated levels in patients with metabolic diseases associated with hypertriglyceridemia. However, it is still unclear whether CPOA2H may influence lipid metabolism in lipogenic tissues. To verify this, HepG2 hepatocytes and 3T3‐L1 adipocytes were cultured with various concentrations of CPOA2H, and then the expressions of genes associated with lipid metabolism were determined. Incubation with CPOA2H at concentrations found in patients with metabolic diseases enhanced the expression of hepatocyte genes associated with lipid synthesis and release, in particular, the fatty acid synthase gene (nearly 20‐fold increase in the mRNA level). In contrast, incubation with CPOA2H caused the downregulation of most adipocyte genes associated with lipid synthesis, whereas the level of leptin mRNA was increased. These findings suggest that CPOA2H may contribute to hypertriglyceridemia in patients with metabolic diseases, upregulating the expression of hepatocyte genes responsible for lipid synthesis and release.
Author Aleksandra Czumaj
Aleksandra Czumaj,,
-
, Adriana Mika (FCh / DEA / LChER)
Adriana Mika,,
- Laboratory of Chemical Environmental Risks
, Michał Chmielewski
Michał Chmielewski,,
-
, Tomasz Sledzinski
Tomasz Sledzinski,,
-
Journal seriesLipids, ISSN 0024-4201, (A 20 pkt)
Issue year2018
Vol53
No3
Pages345-351
Publication size in sheets0.5
Keywords in Englishadipocyte, cyclopropane fatty acid, fatty acid synthase, hepatocyte, hypertriglyceridemia
DOIDOI:10.1002/lipd.12034
Languageen angielski
Score (nominal)25
ScoreMinisterial score = 20.0, 01-11-2018, ArticleFromJournal
Ministerial score (2013-2016) = 25.0, 01-11-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 1.934 (2) - 2016=2.097 (5)
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