New microginins from cyanobacteria of Greek freshwaters

Sevasti-Kiriaki Zervou , Spyros Gkelis , Triantafyllos Kaloudis , Anastasia Hiskia , Hanna Mazur-Marzec

Abstract

Cyanobacteria can form extensive blooms in water with concurrent production and release of a large number of chemically diverse and bioactive metabolites, including hazardous toxins. Significant number of the metabolites belongs to non-ribosomal peptides, with unique residues, unusual structures and great potential for biotechnological application. The biosynthetic pathways of the peptides generate tens of variants, but only part of them has been identified. Microginins are an understudied class of cyanobacterial linear peptides with a characteristic decanoic acid derivative amino acid residue in their structure. In this study, cyanobacterial blooms and isolated strains from Greek lakes were analyzed for the presence of microginins by liquid chromatography coupled to hybrid triple quadrupole/linear ion trap mass spectrometer (LC-qTRAP MS/MS). Microginin structures were elucidated based on the obtained fragmentation spectra. A large number of microginins occurred in blooms of Greek freshwaters and the most frequently detected were Microginin FR1 (70% of samples), Microginin T1 (52%), Microginin 565B (52%), Microginin T2 (43%), and Microginin 565A (43%). Additionally, nine cyanobacterial strains i.e. Nostoc oryzae, Synechococcus sp., Microcystis aeruginosa, Microcystis viridis, and five Microcystis sp., were found to produce microginins. Thirty-six new microginin structures were characterized out of fifty-one totally detected variants. This is the first time that such a diversity of microginins is reported to be present in water bodies. Results clearly demonstrate the great metabolomic potential of cyanobacteria that inhabit Greek freshwaters and significantly expand the knowledge of cyanobacterial secondary metabolites with regards to the class of microginins.
Author Sevasti-Kiriaki Zervou
Sevasti-Kiriaki Zervou,,
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, Spyros Gkelis
Spyros Gkelis,,
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, Triantafyllos Kaloudis
Triantafyllos Kaloudis,,
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, Anastasia Hiskia
Anastasia Hiskia,,
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, Hanna Mazur-Marzec (FOG/IO/DMarB)
Hanna Mazur-Marzec,,
- Division of Marine Biotechnology
Journal seriesChemosphere, ISSN 0045-6535, e-ISSN 1879-1298, (N/A 100 pkt)
Issue year2020
Vol248
Pages1-30
Publication size in sheets1.45
Article number125961
Referenced article (e.g. original article) Zervou Sevasti-Kiriaki, Kaloudis Triantafyllos, Hiskia Anastasia [et al.]: Fragmentation mass spectra dataset of linear cyanopeptides - microginins, in: Data in Brief, vol. 31, 2020, pp. 1-51, Article number:105825, DOI:10.1016/j.dib.2020.105825
Keywords in Englishmicroginins, linear cyanopeptides, Cyanobacteria, Greek freshwaters, structure elucidation, LC-qTRAP MS/MS
ASJC Classification2700 General Medicine; 1600 General Chemistry; 2304 Environmental Chemistry
DOIDOI:10.1016/j.chemosphere.2020.125961
Languageen angielski
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 09-07-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2019 = 1.635; WoS Impact Factor: 2018 = 5.108 (2) - 2018=5.089 (5)
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