On the use of acridinium indicators for the chemiluminescent determination of the total antioxidant capacity of dietary supplements

Karol Krzymiński , Alexander D. Roshal , Paweł Rudnicki-Velasquez , Krzysztof Żamojć

Abstract

Acridinium salts, due to their chemiluminogenic properties, have found several applications in biomedical analysis as labels and indicators, where the assessment of emission intensity is used for the end‐point detection. This work presents the use of chemiluminescent indicators in the form of selected acridinium esters in order to determine the antioxidant properties of exemplary formulations, namely quercetin, vitamin C and the dietary supplement, Apiextract. The principle of measurements is based on a change in the kinetics of emission decay derived from the acridinium cations in alkaline solutions of hydrogen peroxide in the presence of an antioxidant (the analyte). The proposed system makes a beneficial alternative to related methods, which mostly rely on the assessment of emission efficiency and use the luminometric standard luminol – due to superior parameters of acridinium chemiluminescence, among others ‐ high temporary emission efficiency. The features of the proposed method are manifested by a shorter time period of analysis and lower background signals associated with the environmental influences, as compared to typical approaches. The chromatographic (RP‐HPLC) analyses of the substrates and products generated during chemiluminogenic oxidation of acridinium cations under assay conditions are also presented.
Author Karol Krzymiński (FCh / DPCh / LLR)
Karol Krzymiński,,
- Laboratory of Luminescence Research
, Alexander D. Roshal
Alexander D. Roshal,,
-
, Paweł Rudnicki-Velasquez (FCh / DPCh / LLR)
Paweł Rudnicki-Velasquez,,
- Laboratory of Luminescence Research
, Krzysztof Żamojć (FCh / DGICh / LPCC)
Krzysztof Żamojć,,
- Laboratory of Physicochemistry of Coordination Complexes
Journal seriesLuminescence, ISSN 1522-7235, (A 15 pkt)
Issue year2019
Vol34
No5
Pages512-519
Publication size in sheets0.5
Keywords in Englishacridinium esters, antioxidative properties, chemiluminescence, indicators, reactive oxygen species (ROS)
ASJC Classification1601 Chemistry (miscellaneous); 1304 Biophysics
DOIDOI:10.1002/bio.3629
URL https://doi.org/10.1002/bio.3629
Languageen angielski
Score (nominal)15
ScoreMinisterial score = 15.0, 24-07-2019, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2017 = 0.531; WoS Impact Factor: 2017 = 1.671 (2) - 2017=1.47 (5)
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