Interspecies gynogenesis - a way to avoid contamination with radiation induced paternal chromosome fragments
Konrad Ocalewicz , Stefan Dobosz , Krzysztof Jagiełło , Marcin Polonis
AbstractGynogenetic doubled haploids (DHs) as fully inbred fish are useful in developmental biology and aquaculture research. However, it has been noticed that incomplete UV-induced inactivation of the sperm nuclear DNA may result in “contamination” of the gynogenetic specimens with the radiation-induced chromosome fragments. Presumably, such fragments could be eliminated when gametes used for the induced gynogenesis originate from species whose hybrid offspring are lethal due to the genome incompatibility. In the present research gynogenetic development of rainbow trout (Oncorhynchus mykiss Walbaum, 1792), brook trout (Salvelinus fontinalis Mitchill, 1814) and brown trout (Salmo trutta Linnaeus, 1758) was induced using UV irradiated homologous and heterologous spermatozoa. Eggs inseminated with the irradiated sperm were then subjected to high hydrostatic pressure (HHP) shock to inhibit the first cell cleavage and duplicate haploid sets of maternal chromosomes. Produced DH individuals were cytogenetically studied to confirm their ploidy and to detect any karyotypic abnormalities. Survival of gynogenetic DH embryos developing in eggs inseminated with irradiated homologous and heterologous sperm was comparable. Increased mortality among interspecies gynogenotes was observed after hatching. Cytogenetic analysis of DHs confirmed diploid chromosome complements for the studied egg donor species. No chromosome fragments – residues of the UV irradiated paternal genome were found in cells of interspecies gynogenetic DHs. It might be assumed that individuals with UV-irradiated chromosome fragments from heterologous spermatozoa were preferentially lost that could partially explain lower survival of the interspecies gynogenotes when compared to fish that hatched from eggs activated by the irradiated homologous sperm.
|Journal series||Comparative Cytogenetics, ISSN 1993-0771, (A 15 pkt)|
|Publication size in sheets||0.3|
|Conference||23rd International Colloquium on Animal Cytogenetics and Genomics (ICACG), 09-06-2018 - 12-06-2018, Saint-Petersburg, Rosja|
|License||Journal (articles only); published final; ; with publication|
|Not used for evaluation||yes|
|Score|| = 15.0, 26-03-2019, ArticleFromJournal|
= 15.0, 26-03-2019, ArticleFromJournal
|Publication indicators||: 2016 = 0.804; : 2017 = 1.319 (2) - 2017=1.355 (5)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.