Interspecies gynogenesis - a way to avoid contamination with radiation induced paternal chromosome fragments

Konrad Ocalewicz , Stefan Dobosz , Krzysztof Jagiełło , Marcin Polonis

Abstract

Gynogenetic doubled haploids (DHs) as fully inbred fish are useful in developmental biology and aquaculture research. However, it has been noticed that incomplete UV-induced inactivation of the sperm nuclear DNA may result in “contamination” of the gynogenetic specimens with the radiation-induced chromosome fragments. Presumably, such fragments could be eliminated when gametes used for the induced gynogenesis originate from species whose hybrid offspring are lethal due to the genome incompatibility. In the present research gynogenetic development of rainbow trout (Oncorhynchus mykiss Walbaum, 1792), brook trout (Salvelinus fontinalis Mitchill, 1814) and brown trout (Salmo trutta Linnaeus, 1758) was induced using UV irradiated homologous and heterologous spermatozoa. Eggs inseminated with the irradiated sperm were then subjected to high hydrostatic pressure (HHP) shock to inhibit the first cell cleavage and duplicate haploid sets of maternal chromosomes. Produced DH individuals were cytogenetically studied to confirm their ploidy and to detect any karyotypic abnormalities. Survival of gynogenetic DH embryos developing in eggs inseminated with irradiated homologous and heterologous sperm was comparable. Increased mortality among interspecies gynogenotes was observed after hatching. Cytogenetic analysis of DHs confirmed diploid chromosome complements for the studied egg donor species. No chromosome fragments – residues of the UV irradiated paternal genome were found in cells of interspecies gynogenetic DHs. It might be assumed that individuals with UV-irradiated chromosome fragments from heterologous spermatozoa were preferentially lost that could partially explain lower survival of the interspecies gynogenotes when compared to fish that hatched from eggs activated by the irradiated homologous sperm.
Publication typeOthers
Author Konrad Ocalewicz (FOG / IO / DMBE)
Konrad Ocalewicz,,
- Department of Marine Biology and Ecology
, Stefan Dobosz
Stefan Dobosz,,
-
, Krzysztof Jagiełło (FOG / IO / DMBE)
Krzysztof Jagiełło,,
- Department of Marine Biology and Ecology
, Marcin Polonis (FOG / IO / DMBE)
Marcin Polonis,,
- Department of Marine Biology and Ecology
Journal seriesComparative Cytogenetics, ISSN 1993-0771, (A 15 pkt)
Issue year2018
No12 (3)
Pages316-316
Publication size in sheets0.3
Conference23rd International Colloquium on Animal Cytogenetics and Genomics (ICACG), 09-06-2018 - 12-06-2018, Saint-Petersburg, Rosja
URL https://compcytogen.pensoft.net/article/27748/download/pdf/
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Not used for evaluationyes
Score (nominal)0
ScoreMinisterial score = 15.0, 23-08-2018, ArticleFromJournal
Ministerial score (2013-2016) = 15.0, 23-08-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 1.151 (2) - 2016=1.335 (5)
Citation count*
Cite
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back