Immune response against HtrA proteases in children with cutaneous mastocytosis

Joanna Renke , Sabina Kędzierska-Mieszkowska , Magdalena Lange , Bogusław Nedoszytko , Anna Liberek , Katarzyna Plata-Nazar , Marcin Renke , Tomasz Wenta , Dorota Żurawa-Janicka , Joanna Skórko-Glonek , Barbara Lipińska

Abstract

Mast cells play an important role in both, the innate and adaptive immunity, however, clonal proliferation of abnormal mast cells in various organs leads to mastocytosis. A skin variant of the disease, cutaneous mastocytosis (CM) is the most frequent form of mastocytosis in children. HtrA proteases are modulators of important cellular processes, including cell signaling and apoptosis, and are related to development of several pathologies. The above and the observation that mast cells constitutively release the HtrA1 protein, prompted us to investigate a possible involvement of the HtrA proteins in pediatric CM. Levels of the serum autoantibodies (IgG) against the recombinant HtrA proteins (HtrA1-4) in children with CM (n=36) and in healthy controls (n=62) were assayed. Anti-HtrA IgGs were detected using enzyme linked immunosorbent assay (ELISA) and Western-blotting. In the CM sera, levels of the anti-HtrA1 and anti-HtrA3 autoantibodies were significantly increased when compared to the control group, while the HtrA protein levels were comparable. No significant differences in the anti-HtrA2 IgG level were found; for the anti-HtrA4 IgGs lower levels in CM group were revealed. In healthy children, the IgG levels against the HtrA1, -3 and -4 increased significantly with the age of children; no significant changes were observed for the anti-HtrA2 IgG. Our results suggest involvement of the HtrA1 and HtrA3 proteins in pediatric CM; involvement of the HtrA4 protein is possible but needs to be investigated further. In healthy children, the autoantibody levels against HtrA1, -3 and -4, but not against HtrA2, increase with age.
Author Joanna Renke (FB / DB)
Joanna Renke,,
- Department of Biochemistry
, Sabina Kędzierska-Mieszkowska (FB / DB)
Sabina Kędzierska-Mieszkowska,,
- Department of Biochemistry
, Magdalena Lange - [Gdanski Uniwersytet Medyczny]
Magdalena Lange,,
-
- Gdanski Uniwersytet Medyczny
, Bogusław Nedoszytko - [Gdanski Uniwersytet Medyczny]
Bogusław Nedoszytko,,
-
- Gdanski Uniwersytet Medyczny
, Anna Liberek - [Gdanski Uniwersytet Medyczny]
Anna Liberek,,
-
- Gdanski Uniwersytet Medyczny
, Katarzyna Plata-Nazar - [Gdanski Uniwersytet Medyczny]
Katarzyna Plata-Nazar,,
-
- Gdanski Uniwersytet Medyczny
, Marcin Renke - [Gdanski Uniwersytet Medyczny]
Marcin Renke,,
-
- Gdanski Uniwersytet Medyczny
, Tomasz Wenta (FB / DB)
Tomasz Wenta,,
- Department of Biochemistry
, Dorota Żurawa-Janicka (FB / DB)
Dorota Żurawa-Janicka,,
- Department of Biochemistry
, Joanna Skórko-Glonek (FB / DB)
Joanna Skórko-Glonek,,
- Department of Biochemistry
et al.`
Journal seriesActa Biochimica Polonica, ISSN 0001-527X, e-ISSN 1734-154X, (A 15 pkt)
Issue year2018
Vol65
No3
Pages471-478
Publication size in sheets0.5
Keywords in EnglishHtrA proteases, children, cutaneous mastocytosis, mast cells
ASJC Classification1300 General Biochemistry, Genetics and Molecular Biology
DOIDOI:10.18388/abp.2018_2623
URL https://doi.org/10.18388/abp.2018_2623
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa - Na Tych Samych Warunkach (CC-BY-SA); with publication
Score (nominal)15
Score sourcejournalList
ScoreMinisterial score = 15.0, 27-03-2020, ArticleFromJournal
Publication indicators WoS Citations = 0; Scopus Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.64; WoS Impact Factor: 2018 = 1.626 (2) - 2018=1.522 (5)
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