Immune response against HtrA proteases in children with cutaneous mastocytosis

Joanna Renke , Sabina Kędzierska-Mieszkowska , Magdalena Lange , Bogusław Nedoszytko , Anna Liberek , Katarzyna Plata-Nazar , Marcin Renke , Tomasz Wenta , Dorota Żurawa-Janicka , Joanna Skórko-Glonek , Barbara Lipińska


Mast cells play an important role in both, the innate and adaptive immunity, however, clonal proliferation of abnormal mast cells in various organs leads to mastocytosis. A skin variant of the disease, cutaneous mastocytosis (CM) is the most frequent form of mastocytosis in children. HtrA proteases are modulators of important cellular processes, including cell signaling and apoptosis, and are related to development of several pathologies. The above and the observation that mast cells constitutively release the HtrA1 protein, prompted us to investigate a possible involvement of the HtrA proteins in pediatric CM. Levels of the serum autoantibodies (IgG) against the recombinant HtrA proteins (HtrA1-4) in children with CM (n=36) and in healthy controls (n=62) were assayed. Anti-HtrA IgGs were detected using enzyme linked immunosorbent assay (ELISA) and Western-blotting. In the CM sera, levels of the anti-HtrA1 and anti-HtrA3 autoantibodies were significantly increased when compared to the control group, while the HtrA protein levels were comparable. No significant differences in the anti-HtrA2 IgG level were found; for the anti-HtrA4 IgGs lower levels in CM group were revealed. In healthy children, the IgG levels against the HtrA1, -3 and -4 increased significantly with the age of children; no significant changes were observed for the anti-HtrA2 IgG. Our results suggest involvement of the HtrA1 and HtrA3 proteins in pediatric CM; involvement of the HtrA4 protein is possible but needs to be investigated further. In healthy children, the autoantibody levels against HtrA1, -3 and -4, but not against HtrA2, increase with age.
Author Joanna Renke (FB / DB)
Joanna Renke,,
- Department of Biochemistry
, Sabina Kędzierska-Mieszkowska (FB / DB)
Sabina Kędzierska-Mieszkowska,,
- Department of Biochemistry
, Magdalena Lange
Magdalena Lange,,
, Bogusław Nedoszytko
Bogusław Nedoszytko,,
, Anna Liberek
Anna Liberek,,
, Katarzyna Plata-Nazar
Katarzyna Plata-Nazar,,
, Marcin Renke
Marcin Renke,,
, Tomasz Wenta (FB / DB)
Tomasz Wenta,,
- Department of Biochemistry
, Dorota Żurawa-Janicka (FB / DB)
Dorota Żurawa-Janicka,,
- Department of Biochemistry
, Joanna Skórko-Glonek (FB / DB)
Joanna Skórko-Glonek,,
- Department of Biochemistry
et al.`
Journal seriesActa Biochimica Polonica, ISSN 0001-527X, e-ISSN 1734-154X, (A 15 pkt)
Issue year2018
Publication size in sheets0.5
Keywords in EnglishHtrA proteases, children, cutaneous mastocytosis, mast cells
Languageen angielski
LicenseJournal (articles only); published final; Other open licence; with publication
Score (nominal)15
ScoreMinisterial score = 15.0, ArticleFromJournal
Ministerial score (2013-2016) = 15.0, ArticleFromJournal
Publication indicators WoS Impact Factor: 2017 = 1.239 (2) - 2017=1.541 (5)
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