Recombinant Flag-tagged E1E2 glycoproteins from three hepatitis C virus genotypes are biologically functional and elicit cross-reactive neutralizing antibodies in mice
Vasil B. Krapchev , Małgorzata Rychłowska , Alicja Chmielewska , Karolina Zimmer , Arvind H. Patel , Krystyna Bieńkowska-Szewczyk
AbstractHepatitis C virus (HCV) is a globally disseminated human pathogen for which no vaccine is currently available. HCV is highly diverse genetically and can be classified into 7 genotypes and multiple sub-types. Due to this antigenic variation, the induction of cross-reactive and at the same time neutralizing antibodies is a challenge in vaccine production. Here we report the analysis of immunogenicity of recombinant HCV envelope glycoproteins from genotypes 1a, 1b and 2a, with a Flag tag inserted in the hypervariable region 1 of E2. This modification did not affect protein expression or conformation or its capacity to bind the crucial virus entry factor, CD81. Importantly, in immunogenicity studies on mice, the purified E2-Flag mutants elicited high-titer, cross-reactive antibodies that were able to neutralize HCV infectious particles from two genotypes tested (1a and 2a). These findings indicate that E1E2-Flag envelope glycoproteins could be important immunogen candidates for vaccine aiming to induce broad HCV-neutralizing responses.
|Journal series||Virology, ISSN 0042-6822, (A 30 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||hepatitis C virus, E1E2, envelope glycoproteins, Flag tag, neutralization, vaccine|
|License||Other; published final; ; with publication|
|Score|| = 25.0, ArticleFromJournal|
= 30.0, ArticleFromJournal
|Publication indicators||= 1; : 2016 = 0.96; : 2017 = 3.374 (2) - 2017=3.115 (5)|
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