Metabolic engineering of fatty alcohol production in transgenic hairy roots of Crambe abyssinica
Magdalena Miklaszewska , Antoni Banaś , Aleksandra Królicka
AbstractBiotechnological production of fatty alcohols, important raw materials in the chemical industry, has been receiving considerable attention in recent years. Fatty alcohols are formed by the reduction of fatty acyl‐CoAs or fatty acyl‐ACPs catalyzed by a fatty acyl reductase (FAR). In this study, we introduced genes encoding FARs from Arabidopsis thaliana (AtFAR5) and Simmondsia chinensis (ScFAR) into Crambe abyssinica hairy roots via Agrobacterium rhizogenes‐mediated transformation. The efficiency of the transformation ranged between 30 and 45%. The fatty alcohols were only detected in the transgenic hairy root lines expressing ScFAR gene. In all tested lines stearyl alcohol (18:0‐OH), arachidyl alcohol (20:0‐OH), and behenyl alcohol (22:0‐OH) were produced. The content of 18:0‐OH varied from 1 to 3% of total fatty acids and fatty alcohols, while the amount of either 20:0‐OH and 22:0‐OH did not exceed 2%. The transgenic hairy root lines produced from 0.98 to 2.59 nmol of fatty alcohols per mg of dry weight. Very low activity of ScFAR was detected in the microsomal fractions isolated from the selected hairy root lines. To our knowledge, this is the first report on the fatty alcohol production in the hairy root cultures.
|Journal series||Biotechnology and Bioengineering, ISSN 0006-3592, (A 40 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||fatty acyl reductase, fatty alcohols, hairy roots, Crambe abyssinica, simmondsia chinensis|
|ASJC Classification||; ;|
|Not used for evaluation||yes|
|Publication indicators||= 0; : 2017 = 1.189; : 2017 = 3.952 (2) - 2017=4.206 (5)|
|Citation count*||3 (2020-05-26)|
|Uwagi||Artykuł został zgłoszony do parametryzacji za lata 2013-2016|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.