Isolation and phenotypic and morphological characterization of the first Podoviridae lytic bacteriophages ΦA38 and ΦA41 infecting Pectobacterium parmentieri (former Pectobacterium wasabiae)

Anna Smolarska , Łukasz Rąbalski , Magdalena Narajczyk , Robert Czajkowski

Abstract

Two bacteriophages, ϕA38 and ϕA41, infecting Pectobacterium parmentieri strain SCC 3193 (former Pectobacterium wasabiae strain SCC 3193) were isolated from arable soil samples collected in different regions of Poland. ϕA38 and ϕA41 have a typical morphology of the members of the family Podoviride and order Caudovirales, with a head diameter of ca. 60 nm and tail length of ca. 20 nm. Phages ϕA38 and ϕA41 exhibited a similar RFLP pattern with Csp6I restriction endonuclease. They were stable in a range of pHs, temperatures and osmolarities but were rapidly inactivated by UV light. During the first 20 min., 74 and 69% of ϕA38 and ϕA41 phages, respectively, were adsorbed to SCC 3193 cells. In one-step growth experiments, ϕA38 and ϕA41 showed latent period of ca. 20–30 min and burst size of 102 and 141 phages, respectively. The optimal multiplicity of infection (MOI) was calculated to be 0.01 for both bacteriophages. In the host range experiments, both phages were able to infect six from 21 of the tested P. parmentieri isolates but the phages were unable to infect other members of the Pectobacterium spp. or Dickeya spp. In the proof-of-concept experiments, ϕA38 and ϕA41 were able to inhibit the growth of P. parmentieri strain SCC 3193 and to protect potato tuber tissue maceration caused by the bacterium. The potential use of ϕA38 and ϕA41 bacteriophages for the biocontrol of P. parmentieri in potato is discussed.
Author Anna Smolarska (IFB / IB / DBiotech)
Anna Smolarska,,
- Department of Biotechnology
, Łukasz Rąbalski (IFB / IB / LRV)
Łukasz Rąbalski,,
- Laboratory of Recombinant Vaccines
, Magdalena Narajczyk (FB / LEM)
Magdalena Narajczyk,,
- Laboratory of Electron Microscopy
, Robert Czajkowski (IFB / IB / DBiotech)
Robert Czajkowski,,
- Department of Biotechnology
Journal seriesEuropean Journal of Plant Pathology, ISSN 0929-1873, (A 30 pkt)
Issue year2018
Vol150
No2
Pages413-425
Publication size in sheets0.6
Keywords in Englishbiological control, Erwinia carotovora subsp. wasabiae , survival, stability, application
DOIDOI:10.1007/s10658-017-1289-3
URL https://link.springer.com/content/pdf/10.1007%2Fs10658-017-1289-3.pdf
Languageen angielski
LicenseOther; published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)30
ScoreMinisterial score = 30.0, 26-01-2018, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 26-01-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 1.478 (2) - 2016=1.657 (5)
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