Exploring Baltic Sea cyanobacteria for small-molecule inhibitors of microRNA function: a project description

Paweł Brzuzan , Hanna Mazur-Marzec , Filip Stefaniak , Maciej Woźny , Maciej Florczyk

Abstract

Cyanobacteria constitute a rich source of biologically active and structurally diverse compounds. The pharmacological potential of these compounds resides among others in their ability to control the proliferation and growth of cancer cell lines and potent disease-causing microbial agents. Despite recent scientific advances, the way these compounds interact with the body’s molecular structure are still unclear and science still has to discover how the cyanobacterial metabolites interact with cell structures and how cells react to them. In this project, we will study yet unexamined cyanobacterial metabolites, especially the compounds which act as chemical ligands for microRNA (miRNA) -binding sites, making them promising regulators (inhibitors) of gene networks that are involved in various diseases. We will first develop a stable cell line that constitutively expresses a unique miRNA reporter system. Then, we will conduct a screen on chemical compounds discovered in Baltic cyanobacteria to identify small molecules with inhibitory activity and specificity to MIR92b-3p, which has a significant impact on liver cell behavior in humans. We assume that a successful MIR92b-3p inhibitor will bind to the precursors of MIR92b-3p miRNA, disabling the action of either of the two processing enzymes involved in the biogenesis of any miRNA in a cell (Drosha or Dicer), thus affecting the MIR92b function. The discoveries made with these inhibitory chemical molecules could provide insight into the role of the MIR92 pathway in liver diseases and cancer, and possibly, if promising results appear, they may facilitate a strategy for treating some human diseases in the future.
Author Paweł Brzuzan
Paweł Brzuzan,,
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, Hanna Mazur-Marzec (FOG / IO / DMarB)
Hanna Mazur-Marzec,,
- Division of Marine Biotechnology
, Filip Stefaniak
Filip Stefaniak,,
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, Maciej Woźny
Maciej Woźny,,
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, Maciej Florczyk
Maciej Florczyk,,
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Journal seriesEnvironmental Biotechnology, ISSN 1734-4964, e-ISSN 2391-6095, (B 10 pkt)
Issue year2018
Vol14
No1
Pages1-4
Publication size in sheets0.5
Keywords in English cyanobacteria, luciferase reporter cell line, microRNA, small-molecule inhibitor
DOIDOI:10.14799/ebms301
URL http://www.environmentalbiotechnology.pl/eb_dzialy/eb_online/2018/vol14_1/eb2018,14(1)_ms301.pdf
Languageen angielski
Score (nominal)10
ScoreMinisterial score = 10.0, 30-09-2019, ArticleFromJournal
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