Effect of next-generation sequencing in preimplantation genetic testing on live birth ratio
Joanna Liss , Ewa Pastuszek , Sebastian Pukszta , Eva Hoffmann , Waldemar Kuczynski , Aron Lukaszuk , Krzysztof Łukaszuk
AbstractThe present study analysed live birth ratios in frozen embryo transfer (FET) cycles where embryo ploidy status was determined with preimplantation genetic testing (PGT) using next-generation sequencing (NGS). PGT was performed on trophectoderm cells biopsied at the blastocyst stage. The present prospective cohort study included 112 women undergoing frozen embryo transfer, with NGS PGT. The control group consisted of 85 patients who underwent the IVF procedure with FET planned for a subsequent cycle. The live birth rate per cycle was higher by ~18.5 percentage points in the investigated compared with control group (42.0% vs 23.5% respectively; P = 0.012). The differences between the study and control groups were also significant for clinical pregnancy (42.0% vs 23.5% respectively; P = 0.012), implantation (41.2% vs 22.2% respectively; P = 0.001) and pregnancy loss rates (9.6% vs 28.6% respectively; P = 0.027). The results show that PGT NGS is a useful method for embryo selection and it may be implemented in routine clinical practice with propitious results.
|Journal series||Reproduction Fertility and Development, ISSN 1031-3613, (A 25 pkt)|
|Score|| = 25.0, 28-10-2018, ArticleFromJournal|
= 30.0, 28-10-2018, ArticleFromJournal
|Publication indicators||: 2016 = 2.656 (2) - 2016=2.652 (5)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.