Effect of next-generation sequencing in preimplantation genetic testing on live birth ratio

Joanna Liss , Ewa Pastuszek , Sebastian Pukszta , Eva Hoffmann , Waldemar Kuczynski , Aron Lukaszuk , Krzysztof Łukaszuk

Abstract

The present study analysed live birth ratios in frozen embryo transfer (FET) cycles where embryo ploidy status was determined with preimplantation genetic testing (PGT) using next-generation sequencing (NGS). PGT was performed on trophectoderm cells biopsied at the blastocyst stage. The present prospective cohort study included 112 women undergoing frozen embryo transfer, with NGS PGT. The control group consisted of 85 patients who underwent the IVF procedure with FET planned for a subsequent cycle. The live birth rate per cycle was higher by ~18.5 percentage points in the investigated compared with control group (42.0% vs 23.5% respectively; P = 0.012). The differences between the study and control groups were also significant for clinical pregnancy (42.0% vs 23.5% respectively; P = 0.012), implantation (41.2% vs 22.2% respectively; P = 0.001) and pregnancy loss rates (9.6% vs 28.6% respectively; P = 0.027). The results show that PGT NGS is a useful method for embryo selection and it may be implemented in routine clinical practice with propitious results.
Publication typeIn press (online first, early view)
Author Joanna Liss (FB / DMBG)
Joanna Liss,,
- Katedra Biologii i Genetyki Medycznej
, Ewa Pastuszek
Ewa Pastuszek,,
-
, Sebastian Pukszta
Sebastian Pukszta,,
-
, Eva Hoffmann
Eva Hoffmann,,
-
, Waldemar Kuczynski
Waldemar Kuczynski,,
-
, Aron Lukaszuk
Aron Lukaszuk,,
-
, Krzysztof Łukaszuk
Krzysztof Łukaszuk,,
-
Journal seriesReproduction Fertility and Development, ISSN 1031-3613, (A 25 pkt)
Issue year2018
Noonline first
DOIDOI:10.1071/RD17428
Languageen angielski
Score (nominal)30
ScoreMinisterial score = 25.0, 28-10-2018, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 28-10-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 2.656 (2) - 2016=2.652 (5)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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