Metody chemicznej ligacji w syntezie peptydów i białek - część II

Katarzyna Jędrzejewska , Magdalena Kropidłowska , Ewa Wieczerzak

Abstract

Proteins are synthesized only by living organisms. Today, we are able to receive them by recombinant protein expression in bacterial cells. This technique is very useful and gives satisfactory amount of desirable material but it precludes the possibility of introduction of some chemical modifications that are often obligatory. For this reason, chemical synthesis of longer peptide chains is still important and is the object of scientists attention. Over the last century, notion of peptide synthesis took a new meaning. Nowadays, we know a number of innovative methods and also automated devices which help us to make progress in this area. Nevertheless, the synthesis of longer, more complicated peptide chains and proteins still constitutes a problem. Native chemical ligation (NCL) has facilitated the synthesis of numerous complex peptide and protein targets. Expansion of ligation techniques has allowed the entry of peptides into the world of therapeutic drugs [1]. NCL reactions are carried out in aqueous solution and give good yields. Due to mild conditions, NCL overcomes racemic and solubility problems encountered in classical peptide synthesis using protected fragments. The challenge is to synthesize the C-terminal thioester-containing peptide necessary for the transesterification reaction, which is the first step of linking the peptide fragments [2]. In this review we discuss the evolution, advantages and potential applications of chemical ligation reactions. In the first part of this article we described the utility of native chemical ligation approach to non-cysteine containing peptides. This part details a number of important approaches to the synthesis of peptides bearing a C-terminal thioester. Contemporary applications of these techniques to the total chemical synthesis of proteins are also presented.
Author Katarzyna Jędrzejewska PChM
Katarzyna Jędrzejewska,,
- Laboratory of Medical Chemistry
, Magdalena Kropidłowska PChM
Magdalena Kropidłowska ,,
- Laboratory of Medical Chemistry
, Ewa Wieczerzak PChM
Ewa Wieczerzak,,
- Laboratory of Medical Chemistry
Other language title versionsChemical ligation methods in the synthesis of peptides and proteins - part II
Journal seriesWiadomości Chemiczne, ISSN 0043-5104, e-ISSN 2300-0295
Issue year2017
Vol71
No9-10
Pages747-761
Publication size in sheets0.7
Keywords in Polishnatywna chemiczna ligacja, synteza peptydów, tioester
Keywords in Englishnative chemical ligation, peptide synthesis, thioester
URL http://wiad.chem.uni.wroc.pl/files/WCh_9-10-2017_DBC3.pdf
Languagepl polski
LicenseOther; published final; Other open licence; with publication
Score (nominal)7
ScoreMinisterial score = 7.0, 26-02-2018, ArticleFromJournal
Ministerial score (2013-2016) = 7.0, 26-02-2018, ArticleFromJournal
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