First insight into microbiome profile of fungivorous thrips Hoplothrips carpathicus (Insecta: Thysanoptera) at different developmental stages: molecular evidence of Wolbachia endosymbiosis

Agnieszka Kaczmarczyk , Halina Kucharczyk , Marek Kucharczyk , Przemysław Kapusta , Jerzy Sell , Sylwia Zielińska

Abstract

Insects’ exoskeleton, gut, hemocoel, and cells are colonized by various microorganisms that often play important roles in their host life. Moreover, insects are frequently infected by vertically transmitted symbionts that can manipulate their reproduction. The aims of this study were the characterization of bacterial communities of four developmental stages of the fungivorous species Hoplothrips carpathicus (Thysanoptera: Phlaeothripidae), verification of the presence of Wolbachia, in silico prediction of metabolic potentials of the microorganisms, and sequencing its mitochondrial COI barcode. Taxonomybased analysis indicated that the bacterial community of H. carpathicus contained 21 bacterial phyla. The most abundant phyla were Proteobacteria, Actinobacteria, Bacterioidetes and Firmicutes, and the most abundant classes were Alphaproteobacteria, Actinobacteria, Gammaproteobacteria and Betaproteobacteria, with different proportions in the total share. For pupa and imago (adult) the most abundant genus was Wolbachia, which comprised 69.95% and 56.11% of total bacterial population respectively. Moreover, similarity analysis of bacterial communities showed that changes in microbiome composition are congruent with the successive stages of H. carpathicus development. PICRUSt analysis predicted that each bacterial community should be rich in genes involved in membrane transport, amino acid metabolism, carbohydrate metabolism, replication and repair processes.
Author Agnieszka Kaczmarczyk (FB / DGB)
Agnieszka Kaczmarczyk,,
- Katedra Genetyki i Biosystematyki
, Halina Kucharczyk
Halina Kucharczyk,,
-
, Marek Kucharczyk
Marek Kucharczyk,,
-
, Przemysław Kapusta
Przemysław Kapusta,,
-
, Jerzy Sell (FB / DGB)
Jerzy Sell,,
- Katedra Genetyki i Biosystematyki
, Sylwia Zielińska (FB / DMGB)
Sylwia Zielińska,,
- Department of Molecular Genetics of Bacteria
Journal seriesScientific Reports, ISSN 2045-2322, (A 40 pkt)
Issue year2018
Vol8
Pages1-13
Publication size in sheets0.6
DOIDOI:10.1038/s41598-018-32747-x
URL https://www.nature.com/articles/s41598-018-32747-x
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)40
ScoreMinisterial score = 40.0, 27-09-2018, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 27-09-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 4.259 (2) - 2016=4.847 (5)
Citation count*
Cite
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back