Recovery of the first full-length genome sequence of a parapoxvirus directly from a clinical sample

Thomas Günter , Ludwig Haas , Malik Alawi , Peter Wohlsein , Jerzy Marks , Adam Grundhoff , Paul Becher , Nicole Fischer

Abstract

We recovered the first full-length poxvirus genome, including the terminal hairpin region, directly from complex clinical material using a combination of second generation short read and third generation nanopore sequencing technologies. The complete viral genome sequence was directly recovered from a skin lesion of a grey seal thereby preventing sequence changes due to in vitro passaging of the virus. Subsequent analysis of the proteins encoded by this virus identified genes specific for skin adaptation and pathogenesis of parapoxviruses. These data warrant the classification of seal parapoxvirus, tentatively designated SePPV, as a new species within the genus Parapoxvirus.
Author Thomas Günter
Thomas Günter,,
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, Ludwig Haas
Ludwig Haas,,
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, Malik Alawi
Malik Alawi,,
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, Peter Wohlsein
Peter Wohlsein,,
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, Jerzy Marks (FOG / IO / HMS)
Jerzy Marks,,
- Hel Marine Station
, Adam Grundhoff
Adam Grundhoff,,
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, Paul Becher
Paul Becher,,
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, Nicole Fischer
Nicole Fischer,,
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Journal seriesScientific Reports, ISSN 2045-2322, (A 40 pkt)
Issue year2017
Vol7
Pages1-8
Publication size in sheets0.5
DOIDOI:10.1038/s41598-017-03997-y
URL https://www.nature.com/articles/s41598-017-03997-y.pdf
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)40
ScoreMinisterial score = 40.0, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, ArticleFromJournal
Publication indicators WoS Impact Factor: 2017 = 4.122 (2) - 2017=4.609 (5)
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