Purified Stx and λ phage initiator O proteins bind specifically to two different origins of replication in vitro
Katarzyna Kozłowska , Joanna Tymecka-Mulik , Grzegorz Węgrzyn
AbstractThe O protein is a crucial factor initiating the DNA replication of lambdoid bacteriophage. Efficient DNA replication of Shiga toxin-converting phage is necessary for effective production of Shiga toxin – main virulence factor of STEC strains. We developed an improved protocol for overproduction, bacterial cell lysis and purification of λO protein. With use of this method we have also isolated O proteins of Stx-phage P27 and 933W that were never purified before. Purified proteins were tested for their DNA binding activity and revealed a sequence specific interactions.
|Journal series||Protein Expression and Purification, ISSN 1046-5928, (A 15 pkt)|
|Publication size in sheets||0.50|
|Keywords in English||protein overproduction, protein purification, initiator O protein, DNA-protein interactions, λ phage, Stx-phage|
|Not used for evaluation||yes|
|Publication indicators||= 0.000; = 0.000; : 2016 = 0.627; : 2017 = 1.338 (2) - 2017=1.406 (5)|
|Uwagi||Artykuł został zgłoszony do parametryzacji za lata 2013-2016|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.