Purified Stx and λ phage initiator O proteins bind specifically to two different origins of replication in vitro
Katarzyna Kozłowska , Joanna Tymecka-Mulik , Grzegorz Węgrzyn
AbstractThe O protein is a crucial factor initiating the DNA replication of lambdoid bacteriophage. Efficient DNA replication of Shiga toxin-converting phage is necessary for effective production of Shiga toxin – main virulence factor of STEC strains. We developed an improved protocol for overproduction, bacterial cell lysis and purification of λO protein. With use of this method we have also isolated O proteins of Stx-phage P27 and 933W that were never purified before. Purified proteins were tested for their DNA binding activity and revealed a sequence specific interactions.
|Journal series||Protein Expression and Purification, ISSN 1046-5928|
|Publication size in sheets||0.5|
|Keywords in English||protein overproduction, protein purification, initiator O protein, DNA-protein interactions, λ phage, Stx-phage|
|Score|| = 15.0, 20-12-2017, ArticleFromJournal|
= 15.0, 20-12-2017, ArticleFromJournal
|Publication indicators||: 2016 = 1.351 (2) - 2016=1.473 (5)|
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