Purified Stx and λ phage initiator O proteins bind specifically to two different origins of replication in vitro

Katarzyna Kozłowska , Joanna Tymecka-Mulik , Grzegorz Węgrzyn


The O protein is a crucial factor initiating the DNA replication of lambdoid bacteriophage. Efficient DNA replication of Shiga toxin-converting phage is necessary for effective production of Shiga toxin – main virulence factor of STEC strains. We developed an improved protocol for overproduction, bacterial cell lysis and purification of λO protein. With use of this method we have also isolated O proteins of Stx-phage P27 and 933W that were never purified before. Purified proteins were tested for their DNA binding activity and revealed a sequence specific interactions.
Author Katarzyna Kozłowska (FB/DMoB)
Katarzyna Kozłowska ,,
- Department of Molecular Biology
, Joanna Tymecka-Mulik (FB/DMoB)
Joanna Tymecka-Mulik ,,
- Department of Molecular Biology
, Grzegorz Węgrzyn (FB/DMoB)
Grzegorz Węgrzyn,,
- Department of Molecular Biology
Journal seriesProtein Expression and Purification, ISSN 1046-5928, (A 15 pkt)
Issue year2017
Publication size in sheets0.50
Keywords in Englishprotein overproduction, protein purification, initiator O protein, DNA-protein interactions, λ phage, Stx-phage
ASJC Classification1305 Biotechnology
URL http://dx.doi.org/10.1016/j.pep.2016.11.002
Languageen angielski
Not used for evaluationyes
Score (nominal)0
Publication indicators Scopus Citations = 0.000; WoS Citations = 0.000; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 0.627; WoS Impact Factor: 2017 = 1.338 (2) - 2017=1.406 (5)
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UwagiArtykuł został zgłoszony do parametryzacji za lata 2013-2016
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