Autoregulation of greA expression relies on GraL rather than on greA promoter region
Maciej Dylewski , Llorenç Fernández-Coll , Bożena Bruhn-Olszewska , Carlos Balsalobre , Katarzyna Potrykus
AbstractGreA is a well-characterized transcriptional factor that acts primarily by rescuing stalled RNA polymerase complexes, but has also been shown to be the major transcriptional fidelity and proofreading factor, while it inhibits DNA break repair. Regulation of greA gene expression itself is still not well understood. So far, it has been shown that its expression is driven by two overlapping promoters and that greA leader encodes a small RNA (GraL) that is acting in trans on nudE mRNA. It has been also shown that GreA autoinhibits its own expression in vivo. Here, we decided to investigate the inner workings of this autoregulatory loop. Transcriptional fusions with lacZ reporter carrying different modifications (made both to the greA promoter and leader regions) were made to pinpoint the sequences responsible for this autoregulation, while GraL levels were also monitored. Our data indicate that GreA mediated regulation of its own gene expression is dependent on GraL acting in cis (a rare example of dual-action sRNA), rather than on the promoter region. However, a yet unidentified, additional factor seems to participate in this regulation as well. Overall, the GreA/GraL regulatory loop seems to have unique but hard to classify properties.
|Journal series||International Journal of Molecular Sciences, ISSN 1422-0067, (N/A 140 pkt)|
|Publication size in sheets||0.75|
|Keywords in English||greA, GraL, autoregulation, sRNA, RNAP, transcription regulation, gene expression, intrinsic terminator|
|ASJC Classification||; ; ; ; ; ; ;|
|License||Repository; published final; ; with publication|
|Score||= 140.0, 28-01-2020, ArticleFromJournal|
|Publication indicators||= 0; : 2018 = 1.224; : 2018 = 4.183 (2) - 2018=4.331 (5)|
|Licencja||Utwór jest udostępniany na licencji Creative Commons Uznanie autorstwa 4.0 Międzynarodowe (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/d|
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