Autoregulation of greA expression relies on GraL rather than on greA promoter region

Maciej Dylewski , Llorenç Fernández-Coll , Bożena Bruhn-Olszewska , Carlos Balsalobre , Katarzyna Potrykus


GreA is a well-characterized transcriptional factor that acts primarily by rescuing stalled RNA polymerase complexes, but has also been shown to be the major transcriptional fidelity and proofreading factor, while it inhibits DNA break repair. Regulation of greA gene expression itself is still not well understood. So far, it has been shown that its expression is driven by two overlapping promoters and that greA leader encodes a small RNA (GraL) that is acting in trans on nudE mRNA. It has been also shown that GreA autoinhibits its own expression in vivo. Here, we decided to investigate the inner workings of this autoregulatory loop. Transcriptional fusions with lacZ reporter carrying different modifications (made both to the greA promoter and leader regions) were made to pinpoint the sequences responsible for this autoregulation, while GraL levels were also monitored. Our data indicate that GreA mediated regulation of its own gene expression is dependent on GraL acting in cis (a rare example of dual-action sRNA), rather than on the promoter region. However, a yet unidentified, additional factor seems to participate in this regulation as well. Overall, the GreA/GraL regulatory loop seems to have unique but hard to classify properties.
Author Maciej Dylewski (FB / DMGB)
Maciej Dylewski ,,
- Department of Molecular Genetics of Bacteria
, Llorenç Fernández-Coll
Llorenç Fernández-Coll,,
, Bożena Bruhn-Olszewska (FB / DMGB)
Bożena Bruhn-Olszewska,,
- Department of Molecular Genetics of Bacteria
, Carlos Balsalobre
Carlos Balsalobre,,
, Katarzyna Potrykus (FB / DMGB)
Katarzyna Potrykus,,
- Department of Molecular Genetics of Bacteria
Journal seriesInternational Journal of Molecular Sciences, ISSN 1422-0067, (N/A 140 pkt)
Issue year2019
Publication size in sheets0.75
Article number5224
Keywords in EnglishgreA, GraL, autoregulation, sRNA, RNAP, transcription regulation, gene expression, intrinsic terminator
ASJC Classification2700 General Medicine; 1312 Molecular Biology; 1503 Catalysis; 1604 Inorganic Chemistry; 1605 Organic Chemistry; 1606 Physical and Theoretical Chemistry; 1607 Spectroscopy; 1706 Computer Science Applications
Languageen angielski
LicenseRepository; published final; Uznanie Autorstwa (CC-BY); with publication
Dylewski_Maciej_Autoregulation_of_greA_2019.pdf 2.68 MB
Score (nominal)140
Score sourcejournalList
ScoreMinisterial score = 140.0, 28-01-2020, ArticleFromJournal
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.224; WoS Impact Factor: 2018 = 4.183 (2) - 2018=4.331 (5)
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LicencjaUtwór jest udostępniany na licencji Creative Commons Uznanie autorstwa 4.0 Międzynarodowe (CC BY 4.0)
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