Evaluation of sample injection precision in respect to sensitivity in capillary electrophoresis using various injection modes

Krzesimir Ciura , Aleksandra Pawelec , Magdalena Buszewska-Forajta , Michal Markuszewski , Joanna Nowakowska , Adam Prahl , Bartosz Wielgomas , Szymon Dziomba


A comparative study was conducted to assess the injection precision in capillary electrophoresis for cationic analytes (arecoline, codeine, papaverine). The precision was measured in respect to methods sensitivity in various injection modes in capillary electrophoresis: standard hydrodynamic injection (3.45 kPa for 6 s), large volume sample stacking (3.45 kPa for 40 s), and field-amplified sample injection (10 kV for 65 s). All measurements were conducted for aqueous solutions of standards to minimize the errors linked to the sample preparation step. The methods were submitted to precision assessment at three concentration levels: at the limit of quantification, three-fold and ten-fold of limit of quantification. The results were compared to those from high-performance liquid chromatography as a reference technique. The field-amplified sample injection method was shown to provide greatest sensitivity (quantification limits down to 4 ng/mL for all three tested compounds) but the lowest precision. High-performance liquid chromatography was established as the most reliable technique (coefficient of variation in all intraday experiments was below 1%). It was also shown that with a use of large volume sample injection technique, similar sensitivity as in high-performance liquid chromatography can be easily reached.
Author Krzesimir Ciura
Krzesimir Ciura,,
, Aleksandra Pawelec
Aleksandra Pawelec,,
, Magdalena Buszewska-Forajta
Magdalena Buszewska-Forajta,,
, Michal Markuszewski
Michal Markuszewski,,
, Joanna Nowakowska
Joanna Nowakowska,,
, Adam Prahl PChP
Adam Prahl,,
- Laboratory of Chemistry of Biopolymers
, Bartosz Wielgomas
Bartosz Wielgomas,,
, Szymon Dziomba
Szymon Dziomba,,
Other language title versions
Journal seriesJournal of Separation Science, ISSN 1615-9306
Issue year2017
Publication size in sheets0.5
Keywords in Englishcapillary electrophoresis, liquid chromatography, repeatability, sensitivity, stacking
URL http://onlinelibrary.wiley.com/doi/10.1002/jssc.201601027/epdf
Languageen angielski
Score (nominal)30
ScoreMinisterial score = 30.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 2.557 (2) - 2016=2.457 (5)
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