Evaluation of sample injection precision in respect to sensitivity in capillary electrophoresis using various injection modes

Krzesimir Ciura , Aleksandra Pawelec , Magdalena Buszewska-Forajta , Michal Markuszewski , Joanna Nowakowska , Adam Prahl , Bartosz Wielgomas , Szymon Dziomba


A comparative study was conducted to assess the injection precision in capillary electrophoresis for cationic analytes (arecoline, codeine, papaverine). The precision was measured in respect to methods sensitivity in various injection modes in capillary electrophoresis: standard hydrodynamic injection (3.45 kPa for 6 s), large volume sample stacking (3.45 kPa for 40 s), and field-amplified sample injection (10 kV for 65 s). All measurements were conducted for aqueous solutions of standards to minimize the errors linked to the sample preparation step. The methods were submitted to precision assessment at three concentration levels: at the limit of quantification, three-fold and ten-fold of limit of quantification. The results were compared to those from high-performance liquid chromatography as a reference technique. The field-amplified sample injection method was shown to provide greatest sensitivity (quantification limits down to 4 ng/mL for all three tested compounds) but the lowest precision. High-performance liquid chromatography was established as the most reliable technique (coefficient of variation in all intraday experiments was below 1%). It was also shown that with a use of large volume sample injection technique, similar sensitivity as in high-performance liquid chromatography can be easily reached.
Author Krzesimir Ciura - [Gdanski Uniwersytet Medyczny]
Krzesimir Ciura,,
- Gdanski Uniwersytet Medyczny
, Aleksandra Pawelec - [Gdanski Uniwersytet Medyczny]
Aleksandra Pawelec,,
- Gdanski Uniwersytet Medyczny
, Magdalena Buszewska-Forajta - [Gdanski Uniwersytet Medyczny]
Magdalena Buszewska-Forajta,,
- Gdanski Uniwersytet Medyczny
, Michal Markuszewski - [Gdanski Uniwersytet Medyczny]
Michal Markuszewski,,
- Gdanski Uniwersytet Medyczny
, Joanna Nowakowska - [Gdanski Uniwersytet Medyczny]
Joanna Nowakowska,,
- Gdanski Uniwersytet Medyczny
, Adam Prahl (FCh / DOCh / LChB)
Adam Prahl,,
- Laboratory of Chemistry of Biopolymers
, Bartosz Wielgomas - [Gdanski Uniwersytet Medyczny]
Bartosz Wielgomas,,
- Gdanski Uniwersytet Medyczny
, Szymon Dziomba - [Gdanski Uniwersytet Medyczny]
Szymon Dziomba,,
- Gdanski Uniwersytet Medyczny
Other language title versions
Journal seriesJournal of Separation Science, ISSN 1615-9306, (A 30 pkt)
Issue year2017
Publication size in sheets0.5
Keywords in Englishcapillary electrophoresis, liquid chromatography, repeatability, sensitivity, stacking
ASJC Classification1506 Filtration and Separation; 1602 Analytical Chemistry
URL http://onlinelibrary.wiley.com/doi/10.1002/jssc.201601027/epdf
Languageen angielski
Score (nominal)30
Score sourcejournalList
ScoreMinisterial score = 30.0, 03-04-2020, ArticleFromJournal
Publication indicators WoS Citations = 2; Scopus Citations = 4; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 0.805; WoS Impact Factor: 2017 = 2.415 (2) - 2017=2.337 (5)
Citation count*5 (2020-03-25)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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