Capillary electrophoresis study of systemin peptides spreading in tomato plant

Piotr Mucha , Jarosław Ruczyński , Michał Dobkowski , Ewelina Backtrog , Piotr Rekowski

Abstract

Systemin (Sys) is an 18-aa plant peptide hormone involved in the regulation of plant’s defensive response. Sys is considered as a fast-spreading systemic wound signal. We developed a simple and rapid CE method to monitor the spreading of Sys peptides through tomato plant. A 1,2,3-triazole-linked AZT-systemin conjugate was designed as a model to study the possibility of translocating small cargo molecules 3’-Azido-2’,3’- dideoxythymidine by systemin. The Sys peptides (Sys, N -propiolyl Sys, and AZT-systemin conjugate) were injected into the stem and leaves of mature tomato plant. Its transporta- tion throughout the plant tissue was traced by CE. The peptides were clearly visible in the crude tomato exudates and an optimum separation was achieved in 25 mM phosphate “buffer” at pH 2.5 and a voltage of 20 kV using uncoated fused silica capillary. CE analysis showed that Sys peptides are well separated from tomato plant exudates ingredients and are stable in tomato stem and leaf exudates for up to 24 h. CE study revealed that the Sys peptides are effectively spreading throughout tomato stem and leaves and the peptides could be directly detected in the crude plant matrixes. The translocation was strongly in- hibited by sodium azide. The results showed that the established CE method can be used to characterize plant peptides spreading under plant physiological conditions.
Publication typeIn press (online first, early view)
Author Piotr Mucha (FCh / DMB / LChBAC)
Piotr Mucha,,
- Laboratory of Chemistry of Biologically Active Compounds
, Jarosław Ruczyński (FCh / DMB / LChBAC)
Jarosław Ruczyński,,
- Laboratory of Chemistry of Biologically Active Compounds
, Michał Dobkowski (FCh / DMB / LChBAC)
Michał Dobkowski,,
- Laboratory of Chemistry of Biologically Active Compounds
, Ewelina Backtrog (FCh / DMB / LChBAC)
Ewelina Backtrog,,
- Laboratory of Chemistry of Biologically Active Compounds
, Piotr Rekowski (FCh / DMB / LChBAC)
Piotr Rekowski,,
- Laboratory of Chemistry of Biologically Active Compounds
Journal seriesElectrophoresis, ISSN 0173-0835, (A 25 pkt)
Issue year2018
Noonline first
Pages1-7
Publication size in sheets0.5
Keywords in EnglishAZT, capillary electrophoresis, click chemistry, plant peptides spreading, systemin
DOIDOI:10.1002/elps.201800206
URL https://doi.org/10.1002/elps.201800206
Languageen angielski
Score (nominal)30
ScoreMinisterial score = 25.0, 02-11-2018, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 02-11-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 2.744 (2) - 2016=2.487 (5)
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