Decreased triacylglycerol content and elevated contents of cell membrane lipids in colorectal cancer tissue: a lipidomic study

Adriana Mika , Alicja Pakiet , Aleksandra Czumaj , Zbigniew Kaczyński , Ivan Liakh , Jarek Kobiela , Adrian Perdyan , Krystian Adrych , Wojciech Makarewicz , Tomasz Śledziński

Abstract

Recent evidence suggests that lipid composition in cancer tissues may undergo multiple alterations. However, no comprehensive analysis of various lipid groups in colorectal cancer (CRC) tissue has been conducted thus far. To address the problem in question, we determined the contents of triacylglycerols (TG), an energetic substrate, various lipids necessary for cell membrane formation, among them phospholipids (phosphatidylcholine, phosphatidylethanolamine), sphingolipids (sphingomyelin) and cholesterol (free, esterified and total), and fatty acids included in complex lipids. 1H-nuclear magnetic resonance (1H-NMR) and gas chromatography-mass spectrometry (GC-MS) were used to analyze the lipid composition of colon cancer tissue and normal large intestinal mucosa from 25 patients. Compared with normal tissue, cancer tissues had significantly lower TG content, along with elevated levels of phospholipids, sphingomyelin, and cholesterol. Moreover, the content of oleic acid, the main component of TG, was decreased in cancer tissues, whereas the levels of saturated fatty acids and polyunsaturated fatty acids (PUFAs), which are principal components of polar lipids, were elevated. These lipidome rearrangements were associated with the overexpression of genes associated with fatty acid oxidation, and the synthesis of phospholipids and cholesterol. These findings suggest that reprogramming of lipid metabolism might occur in CRC tissue, with a shift towards increased utilization of TG for energy production and enhanced synthesis of membrane lipids, necessary for the rapid proliferation of cancer cells.
Author Adriana Mika (FCh/DEA/LChER)
Adriana Mika,,
- Laboratory of Chemical Environmental Risks
, Alicja Pakiet (FCh/DEA/LChER)
Alicja Pakiet,,
- Laboratory of Chemical Environmental Risks
, Aleksandra Czumaj
Aleksandra Czumaj,,
-
, Zbigniew Kaczyński (FCh/DBCh/LSB)
Zbigniew Kaczyński,,
- Laboratory of Structural Biochemistry
, Ivan Liakh
Ivan Liakh,,
-
, Jarek Kobiela
Jarek Kobiela,,
-
, Adrian Perdyan
Adrian Perdyan,,
-
, Krystian Adrych
Krystian Adrych,,
-
, Wojciech Makarewicz
Wojciech Makarewicz,,
-
, Tomasz Śledziński
Tomasz Śledziński,,
-
Journal seriesJournal of Clinical Medicine, ISSN 2077-0383, (N/A 140 pkt)
Issue year2020
Vol9
No4
Pages1-14
Publication size in sheets0.65
Article number1095
Keywords in Englishcolorectal cancer, lipids, polyunsaturated fatty acids, cancer cell proliferation, nuclear magnetic resonance, cell membrane, lipid oxidation
DOIDOI:10.3390/jcm9041095
URL https://doi.org/10.3390/jcm9041095
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)140
Score sourcejournalList
ScoreMinisterial score = 140.0, 30-04-2020, ArticleFromJournal
Publication indicators WoS Impact Factor: 2018 = 5.688 (2)
Citation count*1 (2020-07-29)
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