Decreased triacylglycerol content and elevated contents of cell membrane lipids in colorectal cancer tissue: a lipidomic study
Adriana Mika , Alicja Pakiet , Aleksandra Czumaj , Zbigniew Kaczyński , Ivan Liakh , Jarek Kobiela , Adrian Perdyan , Krystian Adrych , Wojciech Makarewicz , Tomasz Śledziński
AbstractRecent evidence suggests that lipid composition in cancer tissues may undergo multiple alterations. However, no comprehensive analysis of various lipid groups in colorectal cancer (CRC) tissue has been conducted thus far. To address the problem in question, we determined the contents of triacylglycerols (TG), an energetic substrate, various lipids necessary for cell membrane formation, among them phospholipids (phosphatidylcholine, phosphatidylethanolamine), sphingolipids (sphingomyelin) and cholesterol (free, esterified and total), and fatty acids included in complex lipids. 1H-nuclear magnetic resonance (1H-NMR) and gas chromatography-mass spectrometry (GC-MS) were used to analyze the lipid composition of colon cancer tissue and normal large intestinal mucosa from 25 patients. Compared with normal tissue, cancer tissues had significantly lower TG content, along with elevated levels of phospholipids, sphingomyelin, and cholesterol. Moreover, the content of oleic acid, the main component of TG, was decreased in cancer tissues, whereas the levels of saturated fatty acids and polyunsaturated fatty acids (PUFAs), which are principal components of polar lipids, were elevated. These lipidome rearrangements were associated with the overexpression of genes associated with fatty acid oxidation, and the synthesis of phospholipids and cholesterol. These findings suggest that reprogramming of lipid metabolism might occur in CRC tissue, with a shift towards increased utilization of TG for energy production and enhanced synthesis of membrane lipids, necessary for the rapid proliferation of cancer cells.
|Journal series||Journal of Clinical Medicine, ISSN 2077-0383, (N/A 140 pkt)|
|Publication size in sheets||0.65|
|Keywords in English||colorectal cancer, lipids, polyunsaturated fatty acids, cancer cell proliferation, nuclear magnetic resonance, cell membrane, lipid oxidation|
|License||Journal (articles only); published final; ; with publication|
|Score||= 140.0, 30-04-2020, ArticleFromJournal|
|Publication indicators||: 2018 = 5.688 (2)|
|Citation count*||1 (2020-07-29)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.