Morphological, genetic and biological characterisation of a novel alphabaculovirus isolated from Cryptophlebia peltastica (Lepidoptera: Tortricidae)

Tamryn Marsberg , Michael D. Jukes , Martyna Krejmer-Rąbalska , Łukasz Rąbalski , Caroline M. Knox , Sean D. Moore , Martin P. Hill , Bogusław Szewczyk

Abstract

Cryptophlebia peltastica is an agricultural pest of litchis and macadamias in South Africa with phytosanitary status for certain markets. Current control methods rely on chemical, cultural and classical biological control. However, a microbial control option has not been developed. An Alphabaculovirus from C. peltastica was recovered from a laboratory reared colony and morphologically characterised by transmission electron microscopy (TEM). Analysis of occlusion bodies indicated a single NPV (SNPV) varying in size from 421 to 1263 nm. PCR amplification and sequencing of the polh gene region using universal primers followed by BLAST analysis revealed a 93% similarity to a partial polh gene sequence from Epinotia granitalis NPV. Further genetic characterisation involving single restriction endonuclease (REN) digestion of genomic DNA was carried out to generate profiles for comparison against other baculovirus species and potential new isolates of the same virus. The complete genome of the virus was sequenced, assembled and analysed for a more comprehensive genetic analysis. The genome was 115 728 base pairs (bp) in length with a GC content of 37.2%. A total of 126 open reading frames (ORFs) were identified with minimal overlap and no preference in orientation. Bioassays were used to determine the virulence of the NPV against C. peltastica. The NPV was virulent against C. peltastica with an LC50 value of 6.46 × 103 OBs/ml and an LC90 value of 2.46 × 105 OBs/ml, and time mortality ranging between 76.32 h and 93.49 h. This is the first study to describe the isolation and genetic characterisation of a novel SNPV from C. peltastica, which has potential for development into a biopesticide for the control of this pest in South Africa.
Author Tamryn Marsberg
Tamryn Marsberg,,
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, Michael D. Jukes
Michael D. Jukes,,
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, Martyna Krejmer-Rąbalska (IFB)
Martyna Krejmer-Rąbalska,,
- Intercollegiate Faculty of Biotechnology UG
, Łukasz Rąbalski (IFB / IB / LRV)
Łukasz Rąbalski,,
- Laboratory of Recombinant Vaccines
, Caroline M. Knox
Caroline M. Knox,,
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, Sean D. Moore
Sean D. Moore,,
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, Martin P. Hill
Martin P. Hill,,
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, Bogusław Szewczyk (IFB / IB / LRV)
Bogusław Szewczyk,,
- Laboratory of Recombinant Vaccines
Journal seriesJournal of Invertebrate Pathology, ISSN 0022-2011, (A 40 pkt)
Issue year2018
Vol157
Pages90-99
Publication size in sheets0.5
Keywords in EnglishTortricidae, NPV, litchi moth, genome analysis, restriction analysis, biological control
DOIDOI:10.1016/j.jip.2018.08.006
URL https://doi.org/10.1016/j.jip.2018.08.006
Languageen angielski
Score (nominal)40
ScoreMinisterial score = 40.0, 25-10-2018, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 25-10-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 2.379 (2) - 2016=2.559 (5)
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