Isolation of atropine and scopolamine from plant material using liquid-liquid extraction and EXtrelut & reg columns

Paula Śramska , Artur Maciejka , Anna Topolewska , Piotr Stepnowski , Łukasz Haliński

Abstract

Tropane alkaloids are toxic secondary metabolites produced by Solanaceae plants. Among them, plants from Datura genus produce significant amounts of scopolamine and hyoscyamine; the latter undergoes racemization to atropine during isolation. Because of their biological importance, toxic properties and commonly reported food and animal feed contamination by different Datura sp. organs, there is a constant need for reliable methods for the analysis of tropane alkaloids in many matrices. In the current study, three extraction and sample-clean up procedures for the determination of scopolamine and atropine in plant material were compared in terms of their effectiveness and repeatability. Standard liquid-liquid extraction (LLE) and EXtrelut® NT 3 columns were used for the sample clean-up. Combined ultrasound-assisted extraction and 24 h static extraction using ethyl acetate, followed by multiple LLE steps was found the most effective separation method among tested. However, absolute extraction recovery was relatively low and reached 45–67% for atropine and 52–73% for scopolamine, depending on the compound concentration. The same method was also the most effective one for the isolation of target compounds from Datura stramonium leaves. EXtrelut® columns, on the other hand, displayed relatively low effectiveness in isolating atropine and scopolamine from such a complex matrix and hence could not be recommended. The most effective method was also applied to the extraction of alkaloids from roots and stems of D. stramonium. Quantitative analyses were performed using validated method based on gas chromatography with flame ionization detector (GC-FID). Based on the results, the importance of the proper selection of internal standards in the analysis of tropane alkaloids was stressed out.
Author Paula Śramska (FCh / DEA / LChAD)
Paula Śramska,,
- Laboratory of Chemical Analytics and Diagnostics
, Artur Maciejka (FCh / DEA / LChAD)
Artur Maciejka ,,
- Laboratory of Chemical Analytics and Diagnostics
, Anna Topolewska (FCh / DEA / LANC)
Anna Topolewska ,,
- Laboratory of Analysis of Natural Compounds
, Piotr Stepnowski (FCh / DEA / LChER)
Piotr Stepnowski,,
- Laboratory of Chemical Environmental Risks
, Łukasz Haliński (FCh / DEA / LANC)
Łukasz Haliński,,
- Laboratory of Analysis of Natural Compounds
Journal seriesJournal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, ISSN 1570-0232, (A 30 pkt)
Issue year2017
Vol1043
Pages202-208
Publication size in sheets0.5
Keywords in Englishtropane alkaloids, atropine, scopolamine, liquid-liquid extraction, EXtrelut
DOIDOI:10.1016/j.jchromb.2016.09.003
URL http://dx.doi.org/10.1016/j.jchromb.2016.09.003
Languageen angielski
Score (nominal)30
ScoreMinisterial score = 30.0, 22-11-2018, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 22-11-2018, ArticleFromJournal
Publication indicators Scopus Citations = 3; WoS Impact Factor: 2016 = 2.603 (2) - 2016=2.711 (5)
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