Characterization of the molecular chaperone ClpB from the pathogenic spirochaete Leptospira interrogans

Joanna Krajewska , Anna Modrak-Wójcik , Zbigniew J. Arent , Daniel Więckowski , Michal Zolkiewski , Agnieszka Bzowska , Sabina Kędzierska-Mieszkowska

Abstract

Leptospira interrogans is a spirochaete responsible for leptospirosis in mammals. The molecular mechanisms of the Leptospira virulence remain mostly unknown. Recently, it has been demonstrated that an AAA+ chaperone ClpB (a member of the Hsp100 family) from L. interrogans (ClpBLi) is not only essential for survival of Leptospira under the thermal and oxidative stresses, but also during infection of a host. The aim of this study was to provide further insight into the role of ClpB in the pathogenic spirochaetes and explore its biochemical properties. We found that a non-hydrolysable ATP analogue, ATPγS, but not AMP-PNP induces the formation of ClpBLi hexamers and stabilizes the associated form of the chaperone. ADP also induces structural changes in ClpBLi and promotes its self-assembly, but does not produce full association into the hexamers. We also demonstrated that ClpBLi exhibits a weak ATPase activity that is stimulated by κ-casein and poly-lysine, and may mediate protein disaggregation independently from the DnaK chaperone system. Unexpectedly, the presence of E. coli DnaK/DnaJ/GrpE did not significantly affect the disaggregation activity of ClpBLi and ClpBLi did not substitute for the ClpBEc function in the clpB-null E. coli strain. This result underscores the species-specificity of the ClpB cooperation with the co-chaperones and is most likely due to a loss of interactions between the ClpBLi middle domain and the E. coli DnaK. We also found that ClpBLi interacts more efficiently with the aggregated G6PDH in the presence of ATPγS rather than ATP. Our results indicate that ClpB’s importance during infection might be due to its role as a molecular chaperone involved in reactivation of protein aggregates.
Author Joanna Krajewska KBOiM
Joanna Krajewska,,
- Department of Biochemistry
, Anna Modrak-Wójcik
Anna Modrak-Wójcik,,
-
, Zbigniew J. Arent
Zbigniew J. Arent,,
-
, Daniel Więckowski WB
Daniel Więckowski,,
- Faculty of Biology
, Michal Zolkiewski
Michal Zolkiewski,,
-
, Agnieszka Bzowska
Agnieszka Bzowska,,
-
, Sabina Kędzierska-Mieszkowska KBOiM
Sabina Kędzierska-Mieszkowska,,
- Department of Biochemistry
Other language title versions
Journal seriesPlos One, ISSN 1932-6203
Issue year2017
Vol12
No7
Pages1-21
Publication size in sheets1
DOIDOI:10.1371/journal.pone.0181118
URL http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0181118&type=printable
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)40
ScoreMinisterial score = 35.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 2.806 (2) - 2016=3.394 (5)
Citation count*0
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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