Fe-S cluster Hsp70 chaperones : the ATPase cycle and protein interactions

Rafał Dutkiewicz , Małgorzata Nowak , Elizabeth A. Craig , Jarosław Marszałek


Hsp70 chaperones and their obligatory J-protein cochaperones function together in many cellular processes. Via cycles of binding to short stretches of exposed amino acids on substrate proteins, Hsp70/J-protein chaperones not only facilitate protein folding but also drive intracellular protein transport, biogenesis of cellular structures, and disassembly of protein complexes. The biogenesis of iron–sulfur (Fe–S) clusters is one of the critical cellular processes that require Hsp70/J-protein action. Fe–S clusters are ubiquitous cofactors critical for activity of proteins performing diverse functions in, for example, metabolism, RNA/DNA transactions, and environmental sensing. This biogenesis process can be divided into two sequential steps: first, the assembly of an Fe–S cluster on a conserved scaffold protein, and second, the transfer of the cluster from the scaffold to a recipient protein. The second step involves Hsp70/J-protein chaperones. Via binding to the scaffold, chaperones enable cluster transfer to recipient proteins. In eukaryotic cells mitochondria have a key role in Fe–S cluster biogenesis. In this review, we focus on methods that enabled us to dissect protein interactions critical for the function of Hsp70/J-protein chaperones in the mitochondrial process of Fe–S cluster biogenesis in the yeast Saccharomyces cerevisiae.
Author Rafał Dutkiewicz KBMiK
Rafał Dutkiewicz,,
- Department of Molecular and Cellular Biology
, Małgorzata Nowak MWB UG i GUMed
Małgorzata Nowak,,
- Intercollegiate Faculty of Biotechnology UG
, Elizabeth A. Craig
Elizabeth A. Craig,,
, Jarosław Marszałek KBMiK
Jarosław Marszałek,,
- Department of Molecular and Cellular Biology
Other language title versions
Publication size in sheets1.15
Book David Sheila S. (eds.): Fe-S cluster enzymes : part A, Methods in Enzymology, 2017, Elsevier, ISBN 978-0-12-811944-0, 442 p.
Keywords in EnglishJ-protein Hsc20, protein-protein interaction, GST pull-down assay, mitochondria, yeast
Languageen angielski
Score (nominal)5
ScoreMinisterial score = 5.0, 15-12-2017, BookChapterNotSeriesMainLanguages
Publication indicators WoS Impact Factor: 2014 = 2.088 (2) - 2014=2.272 (5)
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